Effects of PI3K inhibitor NVP-BKM120 on acquired resistance to gefitinib of human lung adenocarcinoma H1975 cells

Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban
Yi-chen LiangGang Wu

Abstract

The effects of class I PI3K inhibitor NVP-BKM120 on cell proliferation, cell cycle distribution, cellular apoptosis, phosphorylation of several proteins of the PI3K/AKT signaling pathway and the mRNA expression levels of HIF1-α, VEGF and MMP9 in the acquired gefitinib resistant cell line H1975 were investigated, and whether NVP-BKM120 can overcome the acquired resistance caused by the EGFR T790M mutation and the underlying mechanism were explored. MTT assay was performed to detect the effect of gefitinib, NVP-BKM120, NVP-BKM120 plus 1 μmol/L gefitinib on growth of H1975 cells. The distribution of cell cycle and apoptosis rate of H1975 cells were examined by using flow cytometry. The mRNA expression levels of tumor-related genes such as HIF1-α, VEGF and MMP9 were detected by using real-time quantitative PCR. Western blotting was used to detect the expression level of phosphorylated proteins in the PI3K/AKT signaling pathway, such as Ser473-p-AKT, Ser235/236-p-S6 and Thr70-p-4E-BP1, as well as total AKT, S6 and 4E-BP1. The results showed that the NVP-BKM120 could inhibit the growth of H1975 cells in a concentration-dependent manner, and H1975 cells were more sensitive to NVP-BKM120 than gefitinib (IC50:1.385 vs. 15.09 μmol/L resp...Continue Reading

References

May 16, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·F A ShepherdJ Berille
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·J R Testa, A Bellacosa
Mar 8, 2002·Cellular Signalling·Karleen M Nicholson, Neil G Anderson
Oct 11, 2002·Advances in Cancer Research·Kathleen A Martin, John Blenis
Apr 2, 2003·Journal of Cancer Research and Clinical Oncology·Branislav JeremicNebojsa Nikolic
Dec 24, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David G PfisterUNKNOWN American Society of Clinical Oncology
Mar 17, 2004·Biochimica Et Biophysica Acta·Masahito HanadaBrian A Hemmings
Jun 24, 2004·Seminars in Cell & Developmental Biology·Celeste J RichardsonJohn Blenis
Feb 25, 2005·The New England Journal of Medicine·Susumu KobayashiBalázs Halmos
Jul 19, 2006·Nature Reviews. Genetics·Jeffrey A EngelmanLewis C Cantley
Feb 24, 2007·Nature Reviews. Cancer·Sreenath V SharmaDaniel A Haber
Jun 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Steven B GendreauAlison H Joly
Jul 6, 2007·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Daniel B CostaMark S Huberman
Dec 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·James BeanWilliam Pao
Jan 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Cai-Hong YunMichael J Eck
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jan 1, 2009·International Journal of Cancer. Journal International Du Cancer·Takafumi KuboShinichi Toyooka
Mar 17, 2009·Neuropathology and Applied Neurobiology·A IdbaihJ-Y Delattre
Apr 23, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Saadia A AzizHarriet M Kluger
Jul 9, 2010·CA: a Cancer Journal for Clinicians·Ahmedin JemalElizabeth Ward
Oct 1, 2010·Science Translational Medicine·Silvia BuonamiciMarion Dorsch
Dec 21, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Saadia A AzizHarriet M Kluger

❮ Previous
Next ❯

Citations

Aug 20, 2014·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Sha-Sha RuanGang Wu
Aug 11, 2015·International Immunopharmacology·Belén BlancoMaría Consuelo Del Cañizo
Jul 17, 2018·Oncology Letters·Sureerat PadthaisongWatcharin Loilome

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban
Yan ZhangJian Chen
Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban
Zhang-Peng RenQiao-Xia Tong
© 2021 Meta ULC. All rights reserved