Effects of PI(4,5)P2 concentration on the F-BAR domain membrane binding as revealed by coarse-grained simulations

Proteins
Tatiana B Stanishneva-Konovalova, Olga S Sokolova

Abstract

Bin/Amphyphysin/Rvs (BAR) domain proteins form a key link between membrane remodeling and cytoskeleton dynamics. They are dimers that bind to membranes via electrostatic interactions with different preferences toward negatively charged lipids. In the present article, we examine the interactions of the F-BAR domain of nervous wreck (Nwk) with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2 )-containing membranes using coarse-grained molecular dynamics. We demonstrated PI(4,5)P2 concentration effects, identified the sequence of events that underlies the protein binding and identified amino acids involved in protein-lipid interactions. Our simulations point out the primary role of the basic stretch at the tips of the dimer, which anchors the protein to the membrane and initiates the binding process. When the PI(4,5)P2 concentration is high, the protein stably associates with the membrane by its concave surface or by the opposite side. At low PI(4,5)P2 concentration, the former orientation becomes more favorable; also a state with only one tip bound is observed, due to the weaker attachment and more pronounced association/dissociation events. Our results provide a theoretical model that describes the lipid-binding behavior of Nwk ...Continue Reading

References

Oct 18, 2001·The Journal of Cell Biology·K FarsadP De Camilli
Nov 19, 2003·Neuron·Kimberly R SchuskeErik M Jorgensen
Dec 3, 2003·Science·Brian J PeterHarvey T McMahon
Mar 27, 2004·Current Biology : CB·Joshua Zimmerberg, Stuart McLaughlin
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Dec 2, 2005·Nature·Stuart McLaughlin, Diana Murray
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J R Sheats, H M McConnell
Jun 10, 2006·The EMBO Journal·Jennifer L GallopHarvey T McMahon
Sep 28, 2006·The Journal of Biological Chemistry·Shiro SuetsuguShigeyuki Yokoyama
Jan 11, 2007·The Journal of Chemical Physics·Giovanni BussiMichele Parrinello
Mar 21, 2007·The Journal of Cell Biology·Pieta K MattilaPekka Lappalainen
Jun 16, 2007·The Journal of Physical Chemistry. B·Siewert J MarrinkAlex H de Vries
Mar 11, 2008·Cell·Adam FrostVinzenz M Unger
Jul 12, 2011·Nature Structural & Molecular Biology·Anette PykäläinenPekka Lappalainen
Sep 9, 2011·The Journal of Biological Chemistry·Alexander von der MalsburgDganit Danino
Apr 3, 2012·Journal of Cell Science·Jaeda Coutinho-BuddFranck Polleux
Jun 14, 2013·Molecular Biology of the Cell·Agata N BecalskaAvital A Rodal
Sep 24, 2013·Cell Reports·Hongxia ZhaoPekka Lappalainen
May 2, 2014·Nucleic Acids Research·Marco BiasiniTorsten Schwede
Nov 18, 2015·Journal of Chemical Theory and Computation·Tsjerk A WassenaarSiewert J Marrink
Mar 12, 2013·Journal of Chemical Theory and Computation·César A LópezSiewert J Marrink
Jan 8, 2013·Journal of Chemical Theory and Computation·Djurre H de JongSiewert J Marrink
Sep 8, 2009·Journal of Chemical Theory and Computation·Xavier PerioleMarco A Ceruso
Dec 22, 2015·Cell Reports·Charlotte F KelleyAvital A Rodal
Sep 8, 2016·Proceedings of the National Academy of Sciences of the United States of America·Tatiana B Stanishneva-KonovalovaAvital A Rodal
Jun 12, 2018·Cell·Leonardo Almeida-SouzaHarvey T McMahon

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Sep 5, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Mirsana P Ebrahimkutty, Milos Galic

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