Effects of plasmid growth hormone-releasing hormone treatment during heat stress

DNA and Cell Biology
Patricia A BrownRuxandra Draghia-Akli

Abstract

A gene therapy treatment with plasmid-based growth hormone-releasing hormone (GHRH) delivered by electroporation (EP) was investigated during heat stress; 32 primiparous cows received 2.5 mg of a GHRH-expressing myogenic plasmid (pSP-HV-GHRH), while 20 were designated as controls. Offspring of treated animals showed a reduction in mortality (47%; p < 0.02), and survival from birth to 260 days was dramatically improved (0% mortality vs. 21% in controls) along with an increase in weight gain (p < 0.05). Milk production was increased compared to controls with an average yield gain of 421 kg/cow (p = 0.028). Prolactin (PRL) levels were also significantly increased compared to controls (p < 0.05). The second pregnancy rate was improved by GHRH treatment (53.3% vs. 30.8%). This study shows that the use of plasmid-mediated therapy delivered by EP can maintain health status during periods of heat stress, important for both animals and potentially humans in hot, challenging climates.

References

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Mar 11, 2003·Technology in Cancer Research & Treatment·Ruxandra Draghia-AkliPatricia A Brown
Apr 3, 2003·American Journal of Physiology. Endocrinology and Metabolism·Amir S KhanRuxandra Draghia-Akli
Sep 29, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Patricia A BrownRuxandra Draghia-Akli
Aug 13, 2005·Advances in Genetics·Lluis M MirJulie Gehl
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Aug 16, 2006·DNA and Cell Biology·Marta L FiorottoRuxandra Draghia-Akli
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Citations

Feb 19, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Angela M Bodles-BrakhopRuxandra Draghia-Akli
Mar 2, 2010·Vaccine·Amir S KhanRuxandra Draghia-Akli
Aug 21, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ryan PersonRuxandra Draghia-Akli

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