PMID: 11331671May 2, 2001Paper

Effects of platelet-activating factor on cytokine production by human uterine cervical fibroblasts

Molecular Human Reproduction
T SuganoI Miyakawa

Abstract

Platelet-activating factor (PAF), a lipid that acts as a potent proinflammatory mediator, is involved in several reproductive processes including parturition. To investigate the effects of PAF on expression of various cytokines by cultured human uterine cervical fibroblasts obtained at term prior to labour, Northern blot analyses and enzyme-linked immunosorbent assays were performed. C-PAF, a stable analogue of PAF, increased expression of interleukin-6 and -8 mRNA in a dose-dependent manner (10(-10) to 10(-8) mol/l of C-PAF), and the expression peaked within 4 h. The corresponding protein concentrations were increased in culture media. Monocyte chemoattractant protein-1 mRNA showed marked induction by 10(-8) mol/l of C-PAF; this peaked by 4 h and was followed by an increase in the protein concentration. Another cytokine, RANTES (regulated upon activation, normal T cell expressed and secreted) showed marked mRNA induction by 10(-8) mol/l of C-PAF, and continued to increase in a time-dependent manner until 24 h. The protein concentration was correspondingly increased in the medium. The PAF-induced cytokine production was abolished by co-incubation with WEB 2170, a specific PAF receptor antagonist. PAF may stimulate local product...Continue Reading

References

Apr 1, 1992·American Journal of Obstetrics and Gynecology·Y P ZhuJ M Johnston
May 1, 1992·American Journal of Obstetrics and Gynecology·R OsmersW Kuhn
Nov 1, 1992·American Journal of Obstetrics and Gynecology·T UchiyamaY Mori
Apr 1, 1992·American Journal of Obstetrics and Gynecology·C MorrisM G Elder
Oct 15, 1991·Biochemical and Biophysical Research Communications·R D YeC G Cochrane
Aug 1, 1991·Biochemical Society Transactions·H NagaseG Salvesen
Jan 3, 1991·European Journal of Pharmacology·M D MitchellS L Schiller
Aug 1, 1995·Obstetrics and Gynecology·R G OsmersH Tschesche
Feb 1, 1995·Molecular and Cellular Endocrinology·A AriciM L Casey
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H E BazanN G Bazan
Sep 1, 1993·American Journal of Obstetrics and Gynecology·C G BarclayA A Calder
Dec 1, 1996·The Journal of Allergy and Clinical Immunology·S MatsukuraM Adachi
Apr 1, 1997·European Journal of Obstetrics, Gynecology, and Reproductive Biology·U B KnudsenK Fredens
Nov 5, 1997·Biochemical and Biophysical Research Communications·R W KellyS C Riley
Jul 10, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A AhmedJ Jiang
Oct 3, 1998·American Journal of Obstetrics and Gynecology·Y TanakaI Miyakawa
Jan 14, 1999·Human Reproduction·F C DenisonS C Riley
Mar 24, 2000·Molecular Human Reproduction·M B SennströmA Brauner

❮ Previous
Next ❯

Citations

Mar 14, 2012·Annals of Biomedical Engineering·Elizabeth VargisAnita Mahadevan-Jansen
Oct 27, 2004·Reproductive Biology and Endocrinology : RB&E·Ylva Stjernholm-VladicLena Sahlin
Sep 4, 2008·Reproductive Biology and Endocrinology : RB&E·Lena SahlinGunvor Ekman-Ordeberg
Feb 7, 2009·Journal of Perinatal Medicine·Lynette B MackayRobert E Garfield
Jun 1, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·I Z Mackenzie
Jun 26, 2012·Cytokine & Growth Factor Reviews·Magali RancezRémi Cheynier
Jul 6, 2012·Fertility and Sterility·Erin S BalasubramaniamMona El-Bahrawy
Sep 18, 2004·Clinical and Experimental Immunology·Y SakamotoS C Robson
Jul 28, 2005·Journal of Reproductive Immunology·Yoshiharu SakamotoStephen C Robson
Oct 28, 2003·The American Journal of Pathology·Michal A ElovitzMark Phillippe
Jun 23, 2015·The Journal of Clinical Investigation·Lu GaoCarole R Mendelson
Jan 30, 2015·American Journal of Respiratory and Critical Care Medicine·Armand O BrownCarlos J Orihuela
Dec 31, 2004·Obstetrics and Gynecology·Ambros HuberChristian F Singer
Jan 31, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Amy M MeyerAlvin M Malkinson
Jan 28, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ling ChengKaren M Harnett
Dec 13, 2003·The Journal of Clinical Endocrinology and Metabolism·Hisashi NaraharaIsao Miyakawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.