Effects of platelet-rich plasma on the activity of human menstrual blood-derived stromal cells in vitro

Stem Cell Research & Therapy
Siwen ZhangJichun Tan

Abstract

Human menstrual blood-derived stromal cells (MenSCs) are highly proliferative and show multiple differentiation capacity. The convenience and non-invasiveness make MenSC a novel cell source for regenerative medicine applications. Platelet-rich plasma (PRP) contains abundant growth factors which are beneficial to wound healing. However, the influence of PRP on MenSCs remains elusive. Here, we evaluated the role of PRP in MenSCs proliferation and assessed the effects of PRP on endometrial receptivity regulation in vitro. MenSCs cultured with 10% activated PRP were compared with those cultured with 10% fetal bovine serum (FBS). Differences in cell proliferation, differentiation, and endometrial receptivity-related gene expression were evaluated. Notably, 10% activated PRP significantly promoted MenSCs proliferation and adipogenic/osteogenic differentiation while suppressing apoptosis. Expression of the mesenchymal stem cells (MSCs) marker CD105 and the perivascular markers SUSD2 and CD146 were elevated after PRP treatment. Moreover, short-term PRP stimulation activated the phosphorylation of Akt and signal transducer and activator of transcription 3 (STAT3) pathways, upregulated expression of FoxO1, LIF, and IL1-β, and downregulat...Continue Reading

References

Jan 30, 2002·Implant Dentistry·R E Marx
Nov 17, 2007·Journal of Translational Medicine·Xiaolong MengNeil H Riordan
Jun 5, 2008·Cell Transplantation·Amit N PatelJulie G Allickson
Jun 23, 2010·Journal of Cellular Physiology·Paola BendinelliMaria Alfonsina Desiderio
Jul 16, 2010·Molecular Human Reproduction·Caroline E Gargett, Hirotaka Masuda
Apr 4, 2012·Cell Transplantation·Hirotaka MasudaCaroline E Gargett
Aug 28, 2013·Journal of Assisted Reproduction and Genetics·Saikat Kumar JanaKoel Chaudhury
May 31, 2014·Circulation Research·Harita DharaneeswaranWilliam C Aird
Jun 7, 2014·Frontiers in Immunology·Maroun KhouryFernando E Figueroa
Jul 16, 2014·Tissue Engineering. Part C, Methods·Fatemeh AtashiAli Modarressi
Aug 7, 2014·Journal of Biological Engineering·Javad VerdiAlexander M Seifalian
Jun 16, 2015·BioMed Research International·Antonio MarmottiGiuseppe M Peretti
Oct 5, 2015·Asian Pacific Journal of Tropical Medicine·Jin-Yang ChenCharlie Xiang
Mar 25, 2016·Ophthalmology and Therapy·Francis S Mah, Christine M Sanfilippo
Apr 6, 2016·Current Stem Cell Reports·Elham MasoudiAli Khademhosseini
Jul 28, 2017·Proceedings of the National Academy of Sciences of the United States of America·Oliver W GriffithGunter P Wagner

❮ Previous
Next ❯

Citations

Nov 23, 2018·Stem Cell Research & Therapy·Haining LvHuidong Jia
Jul 30, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Houyi LvJian Xu

❮ Previous
Next ❯

Methods Mentioned

BETA
density-gradient centrifugation
flow cytometry
fluorescence-activated cell sorting
PCR
immunoprecipitation assay
electrophoresis
Flow

Software Mentioned

CellQuest
7500
Image Pro - Plus
Image J
Image Pro Plus
ModFit
GraphPad Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.