Effects of platelets and white blood cells and antiplatelet agent C7E3 (Reopro) on a new test of PAF procoagulant activity of whole blood

Thrombosis Research
G J DespotisJ H Joist

Abstract

This study was designed to evaluate the effects of varying concentrations of platelets, white blood cells (WBC) and Fab fragments of a monoclonal antibody (c7E3, Reopro) directed at the platelet GpIIb-IIIa receptor complex on ACT-based clot ratio values (hemoSTATUS assay) in healthy volunteers. These measurements were made in heparinized whole blood from 10 normal volunteers in which either platelet or WBC concentrations had been varied by differential centrifugation. In addition, blood collected in either heparin or argatroban was incubated with varying concentrations of c7E3 (Reopro). Clot ratio values (%Maximal) in normal blood did not decrease until average platelet counts were less than 50,000. A marked reduction in clot ratios was observed when WBC concentration increased above or decreased below baseline clot ratios within each patient. Strong linear relationships were observed between white cell concentration and clot ratio values when white cell concentrations were either less or greater than baseline values. When argatroban was used as an anticoagulant, inverse relationships were demonstrated between clot ratio values and increasing c7E3 concentration (Ch 3: r = -0.33, Ch4: r = -0.84, Ch5: r = -0.87, Ch 6: r = -0.71)....Continue Reading

References

Sep 1, 1991·Anesthesiology·C S RinderR Hillman
Jan 3, 1991·The New England Journal of Medicine·J N George, S J Shattil
May 1, 1991·The Journal of Clinical Investigation·M SobelD Marques
Oct 1, 1986·The Journal of Clinical Investigation·D C AltieriA M Capitanio
Aug 15, 1986·Thrombosis Research·F FernandezM R Buchanan
Apr 1, 1984·Anesthesia and Analgesia·M T MooreheadB S Bull
Jul 1, 1995·The American Journal of Physiology·D MacconiA Remuzzi
Sep 1, 1993·The American Journal of Physiology·J GabouryP Kubes
Jan 1, 1993·Agents and Actions·G W RaffE J Lazaro
May 1, 1993·The Journal of Thoracic and Cardiovascular Surgery·L C JohnI B Kovacs

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Citations

Jun 11, 2002·Journal of Interventional Cardiology·Christopher M Thompson, Steven R Steinhubl
Oct 20, 1998·American Heart Journal·S D BerkowitzR S Hillman
Jun 23, 1999·Seminars in Thoracic and Cardiovascular Surgery·G J DespotisL T Goodnough
Jan 27, 1998·Circulation·B S Coller
Oct 27, 2004·Anesthesia and Analgesia·Kenichi A TanakaJerrold H Levy
Sep 14, 2007·Acta Anaesthesiologica Scandinavica·J KawasakiK A Tanaka
Aug 31, 2000·The Annals of Thoracic Surgery·G J Despotis, L T Goodnough
Nov 20, 1998·Chest·J I Weitz, J Hirsh
Jun 15, 2004·British Journal of Anaesthesia·K A TanakaJ H Levy

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