Effects of PM_(2. 5) exposure on synaptic plasticity and Wnt/β-catenin pathway in rat hippocampus

Wei sheng yan jiu = Journal of hygiene research
Pengyou ZhouShoufang Jiang

Abstract

To investigate the effects of PM_(2. 5) exposure on the development of synaptic plasticity and Wnt/β-catenin pathway in hippocampus of offspring rats. Healthy 7-week-old SPF SD rats(n=36) mated with a male to female ratio of 2∶1. Pregnant rats were randomly divided into three groups, including control group, low PM_(2. 5) group, and high PM_(2. 5) group, with eight rats in each group. The low and high PM_(2. 5) concentrations in dynamic exposure cabinet were approximately two times and four times higher than the annual average PM_(2. 5) concentration in Tangshan city respectively. The exposure started from pregnant day 0, until postnatal day 21(PND21) of offspring rats. After weaning, the offspring rats continued to be exposed to PM_(2. 5) until PND42. PND21 and PND42 pups were subjected to Morris water maze and new object recognition experiments. Western blot was used to detect post synaptic density-95(PSD-95), synaptophysin(SYN), growth associated protein(GAP-43), glycogen synthase kinase 3β(GSK-3β), β-catenin protein levels and phosphorylation levels of GSK-3β and β-catenin in the hippocampus of offspring rats. Compared with the control group, the learning and memory abilities of the pups of each PM_(2. 5) group were signifi...Continue Reading

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