Effects of polarized and non-polarized red-light irradiation on proliferation of human Wharton's jelly-derived mesenchymal cells

Biochemical and Biophysical Research Communications
Abdolreza BabaeeMassood Ezzatabadipour

Abstract

Light emitting diode (LED) irradiation has recently been introduced as an encouraging strategy for promotion of cell proliferation. Human umbilical cord Wharton's jelly-derived mesenchymal (hUCM) cells are among the most available mesenchymal cells with a promising application in regenerative medicine. The aim of the present study was to examine the effect of polarized (PL) and non-polarized (NPL) red-light emitted by LED on various proliferation properties of hUCM cells. Cell proliferation was assessed 48 h after irradiation of hUCM cells by different energy densities. Cell density increased to a significant level both in PL and NPL irradiation at 0.954 J/cm2 following WST-1 assay. Staining of irradiated and non-irradiated cells with Hoechst after 3 and 6 days revealed an increased proliferation rate in irradiated cells, but the non-irradiated cells proliferated more than irradiated cells at day 9 of cultivation. Similar results were obtained in trypan blue assay. Scratch repair test for 18 h with an interval of 6 h did not reveal a significant difference between irradiated and non-irradiated cells. In addition, CFU-F assay in PL irradiated cells was higher than control when 500 cells/plate was cultivated. Totally, this study ...Continue Reading

Citations

Feb 23, 2020·Journal of Biophotonics·Nicholas TripodiVasso Apostolopoulos
Feb 23, 2020·Tissue Engineering. Part B, Reviews·Ingrid GarzonMiguel A Martin-Piedra
Jan 21, 2021·International Journal of Radiation Biology·Rafał B LewandowskiElżbieta A Trafny
Feb 25, 2021·Oxidative Medicine and Cellular Longevity·Barbara Sampaio Dias Martins MansanoAndrey Jorge Serra
May 15, 2021·BMC Musculoskeletal Disorders·Maryam SaeidinezhadMassood Ezzatabadipour

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