Effects of poloxamer 188 on phospholipid monolayer morphology: an atomic force microscopy study

Langmuir : the ACS Journal of Surfaces and Colloids
Guohui Wu, Ka Yee C Lee

Abstract

P188, a triblock copolymer of the form poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) is known as an effective membrane sealant. Using a dipalmitoylphosphatidylcholine monolayer at the air-water interface to mimic the outer leaflet of the cell membrane, we have examined the interaction between P188 and a phospholipid film. Atomic force microscopy (AFM) was used to investigate the morphological changes in the lipid monolayer induced by P188 insertion upon transferring the lipid/polymer film from the air-water interface onto a solid substrate. Our AFM results confirm that lipid packing regulates poloxamer insertion such that P188 only changes the morphology of a lipid monolayer when the lipid packing density is below that of the insertion threshold. By combining phase imaging and different driving forces in tapping mode AFM, our results further help reveal the distribution of poloxamers in the lipid monolayer with nanometer resolution.

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Citations

Dec 5, 2009·Molecular Pharmaceutics·Tianyi M KrupkaAgata A Exner
Oct 25, 2012·Physical Chemistry Chemical Physics : PCCP·David I FernandezFrances Separovic
May 28, 2011·Plastic and Reconstructive Surgery·Miguel A MedinaWilliam G Austen
Jul 30, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Yuan Yao, Chuanliang Feng
May 28, 2016·The Journal of Physical Chemistry. B·Ardeshir GoliaeiMax L Berkowitz
Aug 12, 2016·ACS Applied Materials & Interfaces·Siyuan Chen, Rongjun Chen
Oct 30, 2009·The Journal of Physical Chemistry. B·Guohui Wu, Ka Yee C Lee
Jun 7, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·François QuirionIsabelle Lévesque
May 3, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Shanghao LiRoger M Leblanc
Nov 10, 2021·Chemical Reviews·Yoo Kyung Go, Cecilia Leal

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