PMID: 6402308Jan 1, 1983Paper

Effects of polyamine limitation on nucleolar development and morphology in early chick embryos

Cell Differentiation
B LöwkvistO Heby

Abstract

Inhibition of polyamine synthesis in early chick embryos by in ovo treatment with DL-alpha-difluoromethylornithine (DFMO), injected beneath the blastoderm after 5 h of incubation, permanently blocks post-gastrular development. After the first day of polyamine limitation, the embryos possess a thickened primitive streak. Further morphogenesis is blocked and the ectoderm and mesoderm are condensed around the streak. There is obvious suppression of nucleolar formation in 24 h DFMO-treated embryos. In the mesoderm obliquely in front of Hensen's node the frequency of nucleolus-possessing cells is only a few percent lower in DFMO-treated than in control embryos. However, in the same area the frequency of mesoderm cells possessing multiple nucleoli is about 50% lower in the polyamine-depleted embryos. At the ultrastructural level, mesoderm cells from 24 h DFMO-treated embryos show a reduction of the fibrillar component of the nucleolus with a resulting segregation of the nucleolar material. Our data indicate that stimulation of polyamine synthesis is an obligatory step in the differentiation of epiblast cells into mesoderm cells.

References

Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H Emanuelsson, O Heby
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G D KuehnR Braun
Nov 9, 1978·Cell and Tissue Research·O Heby, H Emanuelsson
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Citations

Jan 1, 1987·Cell and Tissue Research·B LöwkvistO Heby
Sep 18, 1992·Brain Research. Developmental Brain Research·K BeekerS Pennington
Jun 29, 2004·Reproductive Toxicology·Sônia Pereira LeiteDaniel Eduardo Saul
Aug 1, 1988·Journal of Cellular Physiology·K Gawel-Thompson, R M Greene
Jun 1, 1985·The Journal of Experimental Zoology·B LöwkvistO Heby
Jun 26, 2003·Evolution & Development·Andrew D JohnsonBrian I Crother
Nov 13, 2004·Archives of Insect Biochemistry and Physiology·V V CardosoA K Oliveira

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