PMID: 2510602Nov 15, 1989Paper

Effects of polychlorinated biphenyls on cytochrome P450 induction in the chick embryo hepatocyte culture

Archives of Biochemistry and Biophysics
L E RodmanLarry W Robertson

Abstract

The effects of pure synthetic polychlorinated biphenyl (PCB) congeners on the induction of cytochrome P450 and associated activities were examined in cultured chick embryo hepatocytes. Dose-response effects for the induction of total cytochrome P450 ethoxyresorufin-O-deethylase (EROD) activity, and benzphetamine demethylase (BPDM) activity were studied using 10 selected tetra- to hexachlorinated PCB congeners. These studies revealed that PCBs caused effects in the chick hepatocyte culture different from previously observed effects in rat liver. Based on their effects in chick hepatocytes, the PCBs could be categorized into two groups. The first group (consisting of 3,3',4,4'-PCB, 3,3',4,4',5-PCB, 3,3',4,4',5,5'-PCB, 2',3,3',4,5-PCB, 2,3,3',4,4',5'-PCB, and 2,3,4,4',5-PCB) induced total cytochrome P450 2.4- to 2.9-fold and EROD activity from 1-2 pmol/min/mg protein to 162-247. There was marked variation in potency, but all these congeners had a maximal inducing dose above which cytochrome P450 concentrations and EROD activities declined. BPDM activities were increased only slightly (1.2- to 1.6-fold) at the maximal cytochrome P450 inducing dose. The second group of congeners (consisting of 2,2',4,5,5'-PCB. 2,2',4,4',5,5'-PCB, an...Continue Reading

References

May 1, 1978·Toxicology and Applied Pharmacology·R W MooreS D Aust
Nov 1, 1977·Clinical Pharmacology and Therapeutics·A P Alvares, A Kappas
Feb 14, 1979·Biochemical and Biophysical Research Communications·J F SinclairH L Bonkowsky
Feb 1, 1989·Archives of Biochemistry and Biophysics·D R DuttonA Parkinson
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·A P AlvaresA Kappas
Feb 1, 1974·Toxicology and Applied Pharmacology·J A GoldsteinD L Jue
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J W HamiltonS E Bloom
Jun 1, 1980·Chemico-biological Interactions·A ParkinsonS Safe
Oct 1, 1981·Biochemical Pharmacology·J F SinclairH Bonkowsky
Apr 15, 1982·The Biochemical Journal·J F SinclairH L Bonkowsky
Aug 1, 1955·Biochimica Et Biophysica Acta·E F NEUFELDS P COLOWICK

❮ Previous
Next ❯

Citations

Jan 12, 2000·Journal of Biochemical and Molecular Toxicology·J R Petrulis, N J Bunce
Jan 1, 1991·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·J T BorlakogluR R Dils
Mar 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A LorenzenS W Kennedy
Feb 11, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·R P Gupta, M B Abou-Donia
Sep 15, 2012·Environmental Science & Technology·Izabela Kania-KorwelHans-Joachim Lehmler
Mar 27, 2013·Chemical Research in Toxicology·Ian K LaiLarry W Robertson
May 15, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jing LiuYang Song
Dec 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S I ShedlofskyL W Robertson
Sep 1, 1990·Archives of Biochemistry and Biophysics·P R SinclairJ F Sinclair
Oct 17, 2020·Environmental Science & Technology·Chun-Yun ZhangHans-Joachim Lehmler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.