Effects of polycyclic aromatic hydrocarbons and abiotic stressors on Fundulus grandis cardiac transcriptomics

The Science of the Total Environment
Elizabeth AllmonMaria Sepulveda

Abstract

Following the 2010 Deepwater Horizon oil spill, extensive research has been conducted on the toxicity of oil and polycyclic aromatic hydrocarbons (PAHs) in the aquatic environment. Many studies have identified the toxicological effects of PAHs in estuarine and marine fishes, however, only recently has work begun to identify the combinatorial effect of PAHs and abiotic environmental factors such as hypoxia, salinity, and temperature. This study aims to characterize the combined effects of abiotic stressors and PAH exposure on the cardiac transcriptomes of developing Fundulus grandis larvae. In this study, F. grandis larvae were exposed to varying environmental conditions (dissolved oxygen (DO) 2, 6 ppm; temperature 20, 30 °C; and salinity 3, 30 ppt) as well as to a single concentration of high energy water accommodated fraction (HEWAF) (∑PAHs 15 ppb). Whole larvae were sampled for RNA and transcriptional changes were quantified using RNA-Seq followed by qPCR for a set of target genes. Analysis revealed that exposure to oil and abiotic stressors impacts signaling pathways associated with cardiovascular function. Specifically, combined exposures appear to reduce development of the systemic vasculature as well as strongly impact th...Continue Reading

References

Dec 16, 1998·The Journal of Laboratory and Clinical Medicine·M W PhelanD V Faller
Jan 14, 1999·Genes & Development·J MimuraY Fujii-Kuriyama
Jun 5, 2004·Marine Environmental Research·Wade H PowellMark E Hahn
May 9, 2007·Nucleic Acids Research·Andreas UntergasserJack A M Leunissen
May 10, 2007·The Journal of Experimental Biology·A P Farrell
May 16, 2008·Aquatic Toxicology·Mark G CarlsJohn P Incardona
Jul 6, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Beth M Cleveland, Jason P Evenhuis
Nov 12, 2010·Journal of Exposure Science & Environmental Epidemiology·John P IncardonaNathaniel L Scholz
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Andrew WhiteheadFernando Galvez
Sep 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Richard CamilliJudith Fenwick
Dec 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Marcia K McNuttFrank Shaffer
Apr 11, 2012·Environmental Toxicology and Chemistry·Colleen D GreerKenneth Lee
May 11, 2013·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Chang-Dong ZhuBiao Yan
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·John P IncardonaNathaniel L Scholz
Aug 7, 2014·American Journal of Physiology. Endocrinology and Metabolism·Sue C Bodine, Leslie M Baehr
Nov 22, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Nishad JayasundaraRichard T Di Giulio
Nov 28, 2014·Journal of Applied Physiology·C C de TheijeS E Köhler
Jun 16, 2016·Marine Pollution Bulletin·Jonny BeyerTracy K Collier
Nov 18, 2016·The Journal of Experimental Biology·Adam N KeenTodd E Gillis
Nov 21, 2016·The Science of the Total Environment·Alexis J KhursigaraAndrew J Esbaugh
Apr 18, 2018·Environmental Toxicology and Chemistry·Maria L RodgersMaria S Sepúlveda
Feb 28, 2019·Frontiers in Genetics·Allen Zinkle, Moosa Mohammadi

❮ Previous
Next ❯

Related Concepts