Effects of Polymorphisms in the SjSP-13 Gene of Schistosoma japonicum on Its Diagnostic Efficacy and Immunogenicity

Frontiers in Microbiology
Xindong XuWeiqing Pan

Abstract

Schistosomiasis japonica is one of the most prevalent parasitic diseases in China. The scarcity of effective diagnostic tools is a major factor that contributes to the high prevalence of schistosomiasis japonica. SjSP-13 is a promising serological diagnostic biomarker of the disease. However, it is unclear whether polymorphisms in SjSP-13 affect its diagnostic efficacy and immunogenicity. Here, we found the SjSP-13 gene was highly polymorphic, and all the alleles of the gene were clustered into two clades, clade A and B. SjSP-13.6 and SjSP-13.25, the representative alleles of clade A and B, were produced in Escherichia coli. The diagnostic value of SjSP-13.6 (AUC = 0.983 ± 0.006), was found to be similar to the SjSP-13.25 (AUC = 0.973 ± 0.009) by receiver operating characteristic (ROC) analysis. SjSP-13.6 and SjSP-13.25 have the same specificity (96.7%), while the sensitivity of SjSP-13.6 (90.4%) is slightly but not significantly higher than SjSP-13.25 (85.2%). The combination use of the two alleles (SjSP-13.6/25) didn't increase the diagnostic performance of SjSP-13 as the AUC value of SjSP-13.6/25 is 0.977 ± 0.009, lower than individual SjSP-13.6 (AUC = 0.983 ± 0.006). In addition, we found the immunogenicity of clade A allel...Continue Reading

References

Mar 28, 2001·Parasitology International·C Minggang, F Zheng
Aug 30, 2005·Acta Tropica·Xiao-Nong ZhouJürg Utzinger
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Feb 9, 2008·Emerging Infectious Diseases·Xiao-Nong ZhouYang Hao
Oct 22, 2008·Lancet·Longde WangXiao-Nong Zhou
Mar 21, 2009·Molecular & Cellular Proteomics : MCP·Feng LiuZe-Guang Han
Jul 17, 2009·Nature·UNKNOWN Schistosoma japonicum Genome Sequencing and Functional Analysis Consortium
Jul 17, 2009·Nature·Matthew BerrimanNajib M El-Sayed
Aug 14, 2010·The Lancet Infectious Diseases·Darren J GrayAllen G Ross
May 25, 2011·Molecular and Biochemical Parasitology·Stephanie L HallR Alan Wilson
Jun 18, 2011·Omics : a Journal of Integrative Biology·Alex LoukasPeter J Hotez
Sep 6, 2011·International Journal for Parasitology·Pauline M CupitCharles Cunningham
Jan 17, 2012·Nature Genetics·Neil D YoungRobin B Gasser
Jan 28, 2012·Nature Genetics·Makedonka Mitreva
May 15, 2012·Acta Tropica·David RollinsonJürg Utzinger
Apr 5, 2014·Lancet·Daniel G ColleyCharles H King
Apr 7, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J UtzingerS Knopp
Jul 8, 2015·The Journal of Infectious Diseases·Patrick DriguezDonald P McManus
Jul 16, 2015·Human Vaccines & Immunotherapeutics·Alessandra Ricciardi, Momar Ndao
Aug 19, 2015·Frontiers in Immunology·Rashika El RidiDonald P McManus
Apr 3, 2016·Vaccine·Maureen MerrifieldMaria Elena Bottazzi
Nov 13, 2017·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Marianette T InobayaAllen G Ross
Feb 23, 2018·Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·Zhang Li-JuanZhou Xiao-Nong

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electrophoresis
ELISA
Genome Sequencing
protein array

Software Mentioned

Mega
SPSS
CLUSTAL X
CLUSTA X

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