Effects of Porphyromonas gingivalis LPS and LR12 peptide on TREM-1 expression by monocytes.

Journal of Clinical Periodontology
Marie DubarCatherine Bisson

Abstract

Periodontal disease involves the activation of host immune response, acting not only as defender of periodontal tissues against bacterial aggression but also as mediator of tissue destruction. Triggering receptor expressed on myeloid cells 1 (TREM-1) is an immune receptor that synergizes with Toll-like receptors in amplifying the inflammatory response mediated by microbial molecules. To investigate the role of P. gingivalis lipopolysaccharide (LPS) and the effect of LR12, a TREM-1 inhibitory peptide, on the expression of membrane-bound and soluble form of TREM-1 on human primary monocytes, as well as the production of proinflammatory cytokines. Cells were stimulated with 1 μg/ml of LPS with or without LR12. PCR, flow cytometry and ELISA were used to determine TREM-1 expressions and cytokines release by monocytes. P. gingivalis LPS can induce a significant increase in TREM-1 expression (mRNA, membrane-bound and soluble form, p < 0.001) as well as cytokines (IL-1β, TNFα) and chemokines (IL-8) production by monocytes. This monocytes' activation was partly prevented by LR12. TREM-1 inhibitors such as LR12 could be interesting for the modulation of the excessive inflammatory response that occurs during periodontal disease.

References

May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A BouchonM Colonna
Mar 27, 2003·Periodontology 2000·Catherine M E ChampagneSteven Offenbacher
Mar 31, 2006·The Journal of the American Dental Association·Craig S MillerMark V Thomas
May 26, 2007·Nature Reviews. Immunology·Iain B McInnes, Georg Schett
Oct 2, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·M OrnatowskaR T Sadikot
Dec 11, 2008·Immunologic Research·Adam D Kennedy, Frank R DeLeo
Nov 12, 2009·European Journal of Immunology·Heng ZhengRuxana T Sadikot
Jan 1, 2009·Journal of Innate Immunity·Philipp HaselmayerMarkus P Radsak
Mar 1, 2011·Journal of Clinical Periodontology·Denis F KinaneUNKNOWN Working Group 2 of Seventh European Workshop on Periodontology
Oct 5, 2011·Molecular Immunology·Nagihan BostanciGeorgios N Belibasakis
May 1, 2012·FEMS Immunology and Medical Microbiology·Nagihan Bostanci, Georgios N Belibasakis
May 4, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marc DeriveSébastien Gibot
Oct 31, 2012·Journal of Leukocyte Biology·Rob J W ArtsMihai G Netea
Jul 3, 2013·International Immunopharmacology·Jiangang ZhouJun Shi
Jun 14, 2014·Clinical and Experimental Immunology·M WilliN Bostanci
Nov 5, 2014·Expert Opinion on Therapeutic Patents·Christopher J PelhamDevendra K Agrawal
Apr 5, 2015·Circulation Research·Amir BoufenzerSébastien Gibot
Mar 8, 2016·Archives of Oral Biology·Juliana Santos Bittencourt MenegatCarlos Marcelo Figueredo
Dec 13, 2016·Annals of Translational Medicine·Herbert Bosshart, Michael Heinzelmann
Dec 23, 2016·Journal of the American College of Cardiology·Jeremie JoffreHafid Ait-Oufella
Feb 25, 2017·International Journal of Pharmaceutics·Marianne ParentAriane Boudier
Jul 28, 2017·Journal of Oral Microbiology·Ingar OlsenGeorge Hajishengallis

❮ Previous
Next ❯

Citations

Apr 24, 2019·JDR Clinical and Translational Research·A SilbereisenN Bostanci
May 8, 2020·International Reviews of Immunology·Pedro Henrique Dos Santos DantasHelioswilton Sales-Campos
Sep 10, 2020·Biomolecules·Tram T T NguyenMirim Jin
Feb 2, 2021·Critical Reviews in Microbiology·Amanda de Oliveira MatosHelioswilton Sales-Campos
Dec 11, 2020·Cells·Huifang SunLiling Tang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.