Abstract
The effect of potassium sorbate (K-sorb), a low-toxicity chemical, to control Monilinia spp. was investigated. Preliminary in vitro studies found the MIC of K-sorb for conidial germination and mycelial growth was, respectively, 260 and 1,250 mg/ liter. Immersion of naturally infected peach and nectarine fruit in a solution (15 g/liter) of K-sorb for 120 s reduced brown rot by over 80% in four of five trials. Although treated fruits showed a significant reduction in firmness with respect to the control, they did not reach the overripe stage and retained acceptable quality parameters. In an attempt to elucidate the mechanism of action for K-sorb, the inhibition of enzymatic activity by K-sorb was also tested. In a radial diffusion assay, the addition of K-sorb to agarose reduced polygalacturonase (PG) activity across the concentrations considered. The greatest reduction (54.3%, with respect to the control) was obtained at a sorbate concentration of 15 g/liter. PG kinetic activity of Monilinia laxa observed by a spectrophotometric assay peaked after 40 min in all samples tested. PG activity was significantly higher in the control than in the samples with increased K-sorb concentrations. In conclusion, based on these findings, K-so...Continue Reading
References
Sep 1, 1990·Food Additives and Contaminants·J L Kinderlerer, P V Hatton
Nov 1, 1984·Applied and Environmental Microbiology·J E Lennox, L J McElroy
Jan 1, 1999·Journal of Applied Microbiology·D BraceyP J Coote
Feb 25, 1999·Journal of Applied Microbiology·R J Lambert, M Stratford
Sep 17, 1999·International Journal of Food Microbiology·S Brul, P Coote
Apr 4, 2000·International Journal of Food Microbiology·C A CamposL N Gerschenson
Apr 6, 2002·International Journal of Food Microbiology·Aurelio López-MaloEnrique Palou
May 10, 2002·Pest Management Science·Lluís PalouInmaculada Viñas
May 28, 2002·Journal of Food Protection·M E VenturiniR Oria
Jun 5, 2003·International Journal of Food Microbiology·Anna BonaterraEmilio Montesinos
Aug 1, 1964·Journal of Bacteriology·G K YORK, R H VAUGHN
Feb 1, 1997·Phytopathology·G WangR M Bostock
Apr 15, 2007·Pakistan Journal of Biological Sciences : PJBS·Ismet Yildirim, Binnur Meriçli Yapici
Jan 1, 2007·Plant Disease·Fiorella NeriPaolo Bertolini
Dec 1, 1997·Plant Disease·Dennis A MargosanDelmer J Henson
Apr 1, 1997·Plant Disease·A R BiggsR Nickerson
Nov 1, 1983·Journal of Food Protection·Lloyd B Bullerman