Effects of PPAR-delta agonist and zinc on vaginal smooth muscle cells collagen and tropoelastin production.

International Urogynecology Journal
Peter TakacsCarlos A Medina

Abstract

The objective was to measure the effects of peroxisome proliferator-activated receptor delta (PPAR-delta) agonist and zinc sulfate (ZS) on vaginal smooth muscle cell (SMC) proliferation, tropoelastin production, and collagen production. SMC cultures were performed from vaginal wall biopsies and were incubated with ZS and PPAR-delta agonist GW501516 (GW). Cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Supernatants and cell lysates were collected. Tropoelastin production was measured by Fastin Elastin Assay and collagen was measured with Sircol Collagen Assay. SMC proliferation was similar with 20 μM ZS or 10 nM GW compared to control. Tropoelastin production was significantly increased by 20 μM ZS and by 10 nM GW. Cell culture surface deposited elastin production was significantly increased by 20 μM ZS and by the combination of 20 μM ZS with 10 nM GW, and collagen production was significantly increased by 10 nM GW and by the combination of 20 μM ZS with 10 nM GW. PPAR-delta agonist and ZS increases vaginal SMC tropoelastin and collagen production.

References

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Nov 10, 2011·International Urogynecology Journal·Peter TakacsCarlos A Medina

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Citations

Oct 16, 2019·Menopause : the Journal of the North American Menopause Society·Ferenc FenyvesiPeter Takacs
Apr 25, 2013·Female Pelvic Medicine & Reconstructive Surgery·Peter TakacsCarlos A Medina
May 14, 2019·Journal of Menopausal Medicine·Peter TakacsRobert Poka
Oct 10, 2020·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Anett CsikósIda Miklós
Jan 8, 2021·Biological Trace Element Research·Anett CsikósPeter Takacs
Apr 18, 2020·Menopause : the Journal of the North American Menopause Society·Peter DamjanovichBence Kozma

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