Effects of prebiotic supplementation on the expression of proteins regulating iron absorption in anaemic growing rats

The British Journal of Nutrition
Renata MarcianoMauro Batista de Morais

Abstract

Prebiotics may increase intestinal Fe absorption in anaemic growing rats. The present study evaluated the effects of high-performance (HP) inulin and oligofructose on factors that regulate Fe absorption in anaemic rats during the growth phase. Male Wistar rats aged 21 d of age were fed AIN-93G ration without Fe for 2 weeks to induce Fe-deficiency anaemia. The rats were fed on day 35 a control diet, or a diet with 10 % HP inulin, or a diet with 10 % oligofructose, without Fe supplementation. The animals were euthanised after 2 weeks, and segments of the duodenum, caecum, colon and liver were removed. The expression levels of proteins in the intestinal segments were assessed using Western blotting. The levels of serum, urine and liver hepcidin and the concentrations of IL-10, IL-6 and TNF-α in the caecum, colon and liver were measured using the ELISA test. HP inulin increased the expression of the divalent metal transporter 1 protein in the caecum by 162 % (P= 0·04), and the expression of duodenal cytochrome b reductase in the colon by 136 % (P= 0·02). Oligofructose decreased the expression of the protein ferroportin in the duodenum (P= 0·02), the concentrations of IL-10 (P= 0·044), IL-6 (P= 0·036) and TNF-α (P= 0·004) in the cae...Continue Reading

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Citations

Apr 7, 2016·Best Practice & Research. Clinical Gastroenterology·Rosica Valcheva, Levinus A Dieleman
Dec 1, 2018·The British Journal of Nutrition·Maisa de Lima Correia SilvaMauro Batista de Morais
Jul 2, 2019·Journal of the Science of Food and Agriculture·Cíntia Tomaz Sant' AnaNeuza Maria Brunoro Costa
Nov 14, 2019·American Journal of Physiology. Cell Physiology·Emily A MinorVazhaikkurichi M Rajendran
Sep 16, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jean-Christophe DescheminGaël Nicolas
Dec 13, 2016·European Journal of Nutrition·Tatiana ChristidesPaul Anthony Sharp
Mar 12, 2017·Applied Microbiology and Biotechnology·Archana Somabhai ChaudhariG Naresh Kumar
Jun 22, 2018·Nutrients·Mariane Moreira Ramiro do CarmoElisvânia Freitas Dos Santos

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