Effects of precryopreservation culture on survival of rat islets transplanted after slow cooling and rapid thawing

Cryobiology
G L Warnock, R V Rajotte

Abstract

Despite the frequent use of in vitro tissue culture before islet cryopreservation, no study has evaluated the ability of this procedure to improve the recovery or in vivo function of frozen-thawed islets. To evaluate this, quantities of 2500 Wistar-Furth (WF) rat islets were allocated to each of four groups (n = 8 each): group 1, freshly isolated; group 2, 48 hr in vitro culture; group 3, cryopreservation; group 4, cryopreservation after 48 hr in culture. Islets were frozen slowly at 0.25 degrees C/min and thawed rapidly at 200 degrees C/min. The number of islets recovered after culture or cryopreservation was determined and viability was assessed by measuring weekly indices of plasma glucose (PG), urine glucose (UG), urine volume (UV), and weight after implantation into the portal vein of streptozotocin-diabetic WF recipients. Islet recovery was 97% after culture, 95% after cryopreservation, and 94% after culture-then cryopreservation. After implantation of group 1 and 2 islets, PG was less than 150 mg/dl at 1 week and UG and UV were normal by 1-2 weeks. Group 3 islets restored normoglycemia at 3 weeks and other indices of diabetes were reversed by 4 weeks; group 4 islets restored normoglycemia at 4 weeks and indices returned ...Continue Reading

References

Oct 1, 1976·The British Journal of Surgery·J FergusonI D Johnston
Sep 1, 1987·Diabetes·M G CoulombeR V Rajotte
Apr 1, 1984·World Journal of Surgery·R V RajotteN N Kneteman
Feb 1, 1964·The Journal of Clinical Endocrinology and Metabolism·J A MOORHOUSEN J ROSEN

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Citations

Jan 1, 1993·Transplant International : Official Journal of the European Society for Organ Transplantation·J ForemanM J Taylor
Sep 29, 1999·Transplantation Proceedings·V NacherE Montanya
Jul 23, 1999·Annals of the New York Academy of Sciences·R V Rajotte
Apr 14, 2016·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Di Liu, Feng Pan
Dec 19, 2007·Biomaterials·Carlos A Agudelo, Hiroo Iwata

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