Effects of processing conditions on the rheological behavior of collagen dispersions

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
W Friess, M Schlapp

Abstract

Biomedical collagen preparations are mainly based on liquid aqueous preparations either used directly as injectables or transferred for example into solid implants or porous devices. In all cases the viscosity of the dispersion or solution has to be controlled or adjusted by pH, temperature, collagen concentration or crosslinking. We tested these effects on the rheological and structural properties of collagen fiber dispersions focusing on oscillatory rheometry. With increasing difference between pH and pI viscosity increased and went through a maximum with less rigidity of the fibers. The dispersions acted like a predominantly permanently linked network. This character changed to only partially linked at low collagen concentration due to isolation of the individual fibers. Up to 40 degrees C, temperature is a tool suitable for adjusting viscosity without changes in the network structure. At 50 degrees C, fourier transform - infrared spectroscopy (FT-IR) spectroscopy indicated the transition of the helical into random coil structure. Renaturation of the helices was found upon cooling but atomic force microscopy (AFM) indicated severe temperature induced damages of the fibers. Crosslinking with glutaraldehyde (GTA) leads to an i...Continue Reading

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Citations

Mar 11, 2004·Tissue Engineering·Laxminarayanan KrishnanJames B Hoying
Apr 5, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Zhenhua TianGuoying Li
Nov 15, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·W SchloeglW Friess
Feb 26, 2014·Connective Tissue Research·Zhenhua TianGuoying Li
Jun 30, 2015·International Journal of Biological Macromolecules·Cuicui DingGuoying Li
Nov 5, 2016·International Journal of Biological Macromolecules·Lian DuanJiao Li
Feb 16, 2008·International Journal of Biological Macromolecules·Guoli LaiGuoying Li
Feb 9, 2005·Journal of Biomaterials Science. Polymer Edition·Rebecca Kuntz Willits, Stacy L Skornia
Jun 16, 2016·Chemical Society Reviews·Luis M De Leon RodriguezMargaret A Brimble
Dec 4, 2009·Journal of Liposome Research·Ana González-ParedesAnna Maria Fadda
Mar 20, 2019·Biomedical Engineering Online·Michael Meyer
Dec 15, 2017·Journal of Biomaterials Applications·Lian DuanJiao Li
Jan 11, 2011·International Journal of Biological Macromolecules·Jiheng Li, Guoying Li

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