Effects of prolonged exposure to low dose metformin in thyroid cancer cell lines

Journal of Cancer
Safar KhederSabapathy P Balasubramanian

Abstract

Background: Thyroid cancer is generally associated with an excellent prognosis, but there is significant long-term morbidity with standard treatment. Some sub-types however have a poor prognosis. Metformin, an oral anti-diabetic drug is shown to have anti-cancer effects in several types of cancer (breast, lung and ovarian cancer). The proposed mechanisms include activation of the Adenosine Mono-phosphate-activated Protein Kinase (AMPK) pathway and inhibition of the mTOR pathway (which promotes growth and proliferation). By inhibiting hepatic gluconeogenesis and increasing glucose uptake by muscles, metformin decreases blood glucose and circulating Insulin levels. Aims: Explore the effect of metformin on the growth and proliferation of thyroid cancer cell lines. Methods: The effects of metformin on thyroid cancer cell lines (FTC-133, K1E7, RO82-W-1, 8305C and TT) and normal thyroid follicular cells (Nthy-ori 3-1) were investigated using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay for cell proliferation; clonogenic assays; FACS analysis for apoptosis and cell cycle, H2A.X phosphorylation (γH2AX) assay for DNA repair and scratch assay for cell migration. Results: Metformin inhibited cell prolifera...Continue Reading

Citations

Jan 1, 2019·Thyroid : Official Journal of the American Thyroid Association·Xiaowei HeWei Tang
Jul 22, 2019·Archives of Toxicology·Sabrina HeinzHeidrun Ellinger-Ziegelbauer
Apr 28, 2020·Life Sciences·Bin ZhaoPeng Shang
Aug 8, 2021·European Journal of Medicinal Chemistry·Oleksandr GrytsaiRachid Benhida
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sherin AbdelrahmanJoanna Izabela Lachowicz

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BETA
transfection

Software Mentioned

Cell Quest Pro
Graphpad Prism
FACSDiva
Cytovision

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