PMID: 12759177May 22, 2003Paper

Effects of propofol on the activity of rat glutamate transporter type 3 expressed in Xenopus oocytes: the role of protein kinase C

Neuroscience Letters
Sang-Hwan DoZhiyi Zuo

Abstract

We investigated the effects of propofol on one type of glutamate transporter, excitatory amino acid transporter 3 (EAAT3) and the role of protein kinase C (PKC) in mediating these effects. Rat EAAT3 was expressed in Xenopus oocytes. L-glutamate (30 microM)-induced membrane currents were measured. Propofol increased glutamate-induced inward currents significantly at two tested concentrations (30 and 100 microM) but not at other concentrations. Propofol (30 microM) significantly increased V(max), but not K(m) of EAAT3 for glutamate. The combination of phorbol-12-myrisate-13-acetate (PMA, a PKC activator) and propofol did not increase the responses further compared with PMA or propofol alone. Three PKC inhibitors (staurosporine, calphostin C, and chelerythrine) did not affect basal EAAT3 activity but significantly inhibited the propofol-enhanced EAAT3 activity. Our results suggest that propofol enhances EAAT3 activity at clinically relevant concentrations and PKC may mediate these effects.

References

Feb 1, 1994·Anesthesia and Analgesia·Z Y EbrahimI Awad
May 23, 2002·Neurology·Bernhard WalderMargitta Seeck

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Citations

Apr 23, 2013·European Journal of Pharmacology·Ilsoon SonZhiyi Zuo
May 5, 2010·Neurochemical Research·Xiao-dong ChaoZhou Fei
Dec 17, 2011·European Journal of Pharmacology·Hyo-Seok NaChong-Soo Kim
May 26, 2015·European Journal of Pharmacology·Yang Sook GilHee Jung Baik
May 22, 2009·Expert Opinion on Therapeutic Targets·Lennart BunchAnders A Jensen
May 7, 2009·Neurochemistry International·Fabian KlausFlorian Lang
Apr 5, 2012·Korean journal of anesthesiology·Jae Hee WooHeeseung Lee
Sep 11, 2018·The Journal of International Medical Research·Jin Hyoung ParkByung Seop Shin
Nov 4, 2020·Clinical Science·Simone M R CamargoFrançois Verrey

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