PMID: 11934647Apr 6, 2002Paper

Effects of prostaglandin E2 on gene expression in primary osteoblastic cells from prostaglandin receptor knockout mice

Bone
X LiLawrence G Raisz

Abstract

Recent studies have shown that stimulation of osteoclastogenesis in cocultures of osteoblasts and spleen cells in response to prostaglandin E2 (PGE2) is markedly decreased when the osteoblasts are derived from cells lacking either the EP2 or the EP4 receptor. Induction of osteoclast formation requires upregulation of receptor activator of nuclear factor-kappaB ligand (RANKL) on cells of the osteoblastic lineage, which then binds to the RANK receptor on cells of the osteoclast lineage. Osteoprotegerin (OPG) is a decoy receptor for RANKL that can block its interaction with RANK. In addition, macrophage-colony stimulating factor (M-CSF) is essential for osteoclast formation. Finally, PGE2 can increase interleukin-6 (IL-6), which may further enhance osteoclastogenesis. To study the relative influence of the EP2 and EP4 receptors on response of these factors to PGE2, we examined mRNA levels for RANKL, OPG, M-CSF, and IL-6 in primary osteoblastic cell cultures derived from two lines of EP2 knockout mice (EP2-/-) and one line of EP4 knockout mice (EP4-/-) and the relevant wild-type controls (EP2+/+ and EP4+/+). The responses of cells from wild-type animals of all three lines were similar. After PGE2 treatment, RANKL mRNA levels were i...Continue Reading

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Citations

Dec 12, 2007·Cancer Metastasis Reviews·Karen M BussardAndrea M Mastro
May 16, 2002·Prostaglandins & Other Lipid Mediators·Xiaodong LiLawrence G Raisz
Jun 20, 2003·Experimental Gerontology·Bruce R Troen
Oct 2, 2003·Prostaglandins & Other Lipid Mediators·Lawrence G Raisz, Florence N Woodiel
Jun 10, 2010·Menopause : the Journal of the North American Menopause Society·Yan LuVolodymyr Dvornyk
Oct 26, 2011·Climacteric : the Journal of the International Menopause Society·R PanV Dvornyk
Mar 17, 2010·Biochemical and Biophysical Research Communications·Xin-Hua LiuAlice C Levine
Sep 12, 2009·Prostaglandins & Other Lipid Mediators·Katherine A BlackwellCarol C Pilbeam
Mar 15, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Maxime A GallantArtur J de Brum-Fernandes
Jun 9, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xiaochao WeiRegis J O'Keefe
Feb 6, 2004·Journal of Biomedical Materials Research. Part a·Takaaki FujishiroMasahiro Kurosaka
Apr 24, 2007·Journal of Biomedical Materials Research. Part a·Yebin QianYao Jiang
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Feb 8, 2005·Biochemical and Biophysical Research Communications·Christine A ClarkMichael J Zuscik
Feb 7, 2006·The Journal of Surgical Research·Balraj SinghAnthony Lucci
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May 12, 2009·Expert Opinion on Investigational Drugs·Simon GrahamEleftherios Tsiridis
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Feb 24, 2009·Toxicologic Pathology·Zaher A Radi

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