Effects of prostaglandin F(2alpha)and carbachol on MAP kinases, cytosolic phospholipase A(2)and arachidonic acid release in cat iris sphincter smooth muscle cells

Experimental Eye Research
Shahid Husain, Ata A Abdel-Latif

Abstract

The signal transduction pathways initiated by Ca(2+)-mobilizing agonists, such as prostaglandin F(2alpha)(PGF(2alpha)) and carbachol (CCh), leading to activation of cytosolic phospholipase A(2)(cPLA(2)) and arachidonic acid (AA) release in a wide variety of tissues remain obscure. To further define the role of protein kinases in receptor mediated stimulation of cPLA(2)and consequently AA release we have investigated the role of mitogen-activated protein (MAP) kinases and protein kinase C (PKC) in PGF(2alpha)- and CCh-induced cPLA(2)phosphorylation and AA release in cat iris sphincter smooth muscle (CISM) cells. The cells were prelabeled with [(3)H]AA for 24 hr and incubated in the absence or presence of the agonist for 5-10 min as indicated. MAP kinases activities and cPLA(2)phosphorylation were determined in immunoprecipitates obtained by using anti-p38 MAP kinase and anti-cPLA(2)antibodies. We found that: (a) PGF(2alpha)and CCh increased p38 MAP kinase activity by 197 and 215%, respectively, and increased p42/p44 MAP kinase activity by 200 and 125%, respectively. (b) SB202190, a p38 MAP kinase specific inhibitor, inhibited PGF(2alpha)- and CCh-induced cPLA(2)phosphorylation by 92 and 85%, respectively, and AA release by 62 an...Continue Reading

References

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Citations

Jan 1, 2008·Expert Review of Ophthalmology·Zhou WanW Daniel Stamer
Jul 18, 2015·Veterinary Ophthalmology·Jessica E McDonaldGillian J McLellan
Oct 10, 2002·Biochemical and Biophysical Research Communications·Shahid Husain, Farahdiba Jafri
Aug 4, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Burkhard HinzKay Brune
Feb 11, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Huiping ZhouKarnam S Murthy
Jan 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sarah G HarrisRichard P Phipps

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