PMID: 6809648Jan 1, 1982Paper

Effects of prostaglandins on the development of cell-mediated immunity in culture and on the cytolytic activity of in vivo-generated effector cells

International Journal of Immunopharmacology
K H Leung, E Mihich


This study was undertaken in an attempt to better understand the rôle of prostaglandins in the development of the primary cell-mediated immunity (CMI) response in culture. Primary sensitization cultures with C57Bl/6 mouse spleen cells as responder cells (R) and X-irradiated P815 mastocytoma cells as allogeneic stimulator cells (S) were established in the presence and absence of test agents. The ability of effector cells to lyse P815 target cells was measured by 51Cr-release assay on day 4. 3H-Thymidine uptake into the cultured cells was also analyzed on the same day. Prostaglandins of the E series were shown to exert selective effects on the CMI response depending on dose, schedule of administration, and culture conditions. PGE1 or PGE2 enhanced the CMI response at 30 pM but inhibited it by 50% at 30 nM. At an optimal R/S ratio, 30 nM PGE1, or PGE2 always inhibited 3H-thymidine uptake more than cytotoxicity while at suboptimal ratios it inhibited both equally. PGE1 did not affect the kinetics of development of the CMI responses. PGE1 was inhibitory when added 20 h before, at the same time as or as late as 24 h after antigen. The inhibitory effect was prevented by removal of PGE1 within 24 h of addition. Pretreatment of spleen c...Continue Reading


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