PMID: 6809648Jan 1, 1982Paper

Effects of prostaglandins on the development of cell-mediated immunity in culture and on the cytolytic activity of in vivo-generated effector cells

International Journal of Immunopharmacology
K H Leung, E Mihich

Abstract

This study was undertaken in an attempt to better understand the rôle of prostaglandins in the development of the primary cell-mediated immunity (CMI) response in culture. Primary sensitization cultures with C57Bl/6 mouse spleen cells as responder cells (R) and X-irradiated P815 mastocytoma cells as allogeneic stimulator cells (S) were established in the presence and absence of test agents. The ability of effector cells to lyse P815 target cells was measured by 51Cr-release assay on day 4. 3H-Thymidine uptake into the cultured cells was also analyzed on the same day. Prostaglandins of the E series were shown to exert selective effects on the CMI response depending on dose, schedule of administration, and culture conditions. PGE1 or PGE2 enhanced the CMI response at 30 pM but inhibited it by 50% at 30 nM. At an optimal R/S ratio, 30 nM PGE1, or PGE2 always inhibited 3H-thymidine uptake more than cytotoxicity while at suboptimal ratios it inhibited both equally. PGE1 did not affect the kinetics of development of the CMI responses. PGE1 was inhibitory when added 20 h before, at the same time as or as late as 24 h after antigen. The inhibitory effect was prevented by removal of PGE1 within 24 h of addition. Pretreatment of spleen c...Continue Reading

References

Nov 1, 1979·Nature·D A Stringfellow, F A Fitzpatrick
Oct 1, 1975·The Journal of Experimental Medicine·J NedrudW R Clark
Mar 15, 1977·Life Sciences·L M Pelus, H R Strausser
Sep 1, 1977·Cellular Immunology·D R Webb, I Nowowiejski
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·O J PlesciaK Grinwich
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D R Webb, P L Osheroff
Apr 5, 1974·Science·H R BourneG M Shearer
Aug 1, 1974·The Journal of Clinical Investigation·V A Ferraris, F R DeRubertis
Sep 1, 1967·The Journal of Experimental Medicine·R I Mishell, R W Dutton

Citations

Jan 1, 1988·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M J TarrR H Fertel
Aug 1, 1984·The Journal of Clinical Investigation·S L KunkelG I Higashi
Jan 1, 1988·Immunopharmacology and Immunotoxicology·G P DuffieH T Wepsic
Jan 1, 1989·Immunopharmacology and Immunotoxicology·G R BurlesonJ D Stutzman

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Nodding Syndrome

Nodding Syndrome is a neurological and epileptiform disorder characterized by psychomotor, mental, and growth retardation. Discover the latest research on Nodding Syndrome here.

LRRK2 & Microtubules

Mutations in the LRRK2 gene are risk-factors for developing Parkinson’s disease (PD). LRRK2 mutations in PD have been shown to enhance its association with microtubules. Here is the latest research.