PMID: 7538636Mar 1, 1995Paper

Effects of protein kinase C modulators on multidrug resistance in human glioma cells

Neurosurgery
T MatsumotoK Kaba

Abstract

To identify the role of protein kinase C (PKC) in multidrug resistance, the effects of phorbol-12-myristate-13-acetate (PMA), a PKC activator, or calphostin C, a PKC inhibitor, on intracellular vincristine accumulation and expression of P-glycoprotein phosphorylation were studied in one multidrug-resistant and three multidrug-sensitive human glioma cell lines. Basal PKC activities and immunoreactivities of PKC-alpha and -zeta were higher in multidrug-resistant cells than in multidrug-sensitive cells. There was no significant difference in the immunoreactivity of PKC-delta between multidrug-resistant and -sensitive cells, and immunoreactive PKC-beta, -gamma, and -epsilon were not detected in either multidrug-resistant or -sensitive cells. The treatment of multidrug-resistant cells with 100 nM PMA for 2 hours resulted in the activation not of PKC-zeta but of PKC-alpha, with concomitant decrease in vincristine accumulation and increase in P-glycoprotein phosphorylation. The exposure of multidrug-resistant cells to 100 nM PMA for 24 hours induced down-regulation not of PKC-zeta but of PKC-alpha, with concurrent decrease in vincristine accumulation, and reduced but still increased P-glycoprotein phosphorylation. The treatment of mul...Continue Reading

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Citations

Nov 26, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·K K ChoM L Rosenblum
Feb 18, 2004·Hematology·Alberto Grossi, Monica Biscardi
Mar 1, 2015·Molecular Pharmacology·Mahlet B Abera, Marcelo G Kazanietz
Nov 1, 1995·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·G H BaltuchV W Yong

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