Effects of protein type and composition on postprandial markers of skeletal muscle anabolism, adipose tissue lipolysis, and hypothalamic gene expression

Journal of the International Society of Sports Nutrition
Christopher Brooks MobleyMichael D Roberts

Abstract

We examined the acute effects of different dietary protein sources (0.19 g, dissolved in 1 ml of water) on skeletal muscle, adipose tissue and hypothalamic satiety-related markers in fasted, male Wistar rats (~250 g). Oral gavage treatments included: a) whey protein concentrate (WPC, n = 15); b) 70:30 hydrolyzed whey-to-hydrolyzed egg albumin (70 W/30E, n = 15); c) 50 W/50E (n = 15); d) 30 W/70E (n = 15); and e) 1 ml of water with no protein as a fasting control (CTL, n = 14). Skeletal muscle analyses revealed that compared to CTL: a) phosphorylated (p) markers of mTOR signaling [p-mTOR (Ser2481) and p-rps6 (Ser235/236)] were elevated 2-4-fold in all protein groups 90 min post-treatment (p < 0.05); b) WPC and 70 W/30E increased muscle protein synthesis (MPS) 104% and 74% 180 min post-treatment, respectively (p < 0.05); and c) 70 W/30E increased p-AMPKα (Thr172) 90 and 180-min post-treatment as well as PGC-1α mRNA 90 min post-treatment. Subcutaneous (SQ) and omental fat (OMAT) analyses revealed: a) 70 W/30 W increased SQ fat phosphorylated hormone-sensitive lipase [p-HSL (Ser563)] 3.1-fold versus CTL and a 1.9-4.4-fold change versus all other test proteins 180 min post-treatment (p < 0.05); and b) WPC, 70 W/30E and 50 W/50E incr...Continue Reading

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Citations

Feb 5, 2016·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Lemuel W TaylorKristen Dugan
Dec 18, 2015·Journal of the International Society of Sports Nutrition·Juha J HulmiHeikki Peltonen
Aug 19, 2015·Journal of the International Society of Sports Nutrition·C Brooks MobleyMichael D Roberts
Jan 25, 2019·International Journal of Sport Nutrition and Exercise Metabolism·Fernando NaclerioDiego Moreno-Pérez
Dec 19, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Michael D RobertsAndreas N Kavazis
Mar 28, 2017·Journal of Dairy Science·C Brooks MobleyMichael D Roberts
Sep 27, 2018·Frontiers in Nutrition·Cody T HaunMichael D Roberts
Sep 16, 2020·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Aimee L DordevicDavid Cameron-Smith
Mar 22, 2021·Nutrition·Prasanthi JegatheesanJean-Pascal De Bandt

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Methods Mentioned

BETA
protein treatments
dissection
Protein Assay
dissections
Antibody Array
antibody chip
chip
electrophoresis
PCR

Software Mentioned

SPSS
UVP Imager
SUnSET

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