Effects of Pseudomonas aeruginosa elastase on alveolar epithelial permeability in guinea pigs.

Infection and Immunity
A O AzghaniA R Johnson

Abstract

Elastase-deficient mutants of Pseudomonas aeruginosa are less virulent than the wild type and are easily cleared from the lungs of guinea pigs. The effect of P. aeruginosa elastase on lung epithelium, however, is not yet understood. We addressed the hypothesis that breach of the epithelial barrier by elastase from P. aeruginosa allows invading organisms and toxic substances to penetrate the interstitium. We measured the clearance of aerosolized technetium-labeled albumin (molecular weight, 69,000) from the lungs of anesthetized guinea pigs with the aid of a gamma camera and a dedicated computer. Aerosols of the elastase (0.1 to 5 micrograms) increased the rate of clearance of labeled albumin from the lungs in proportion to the elastase dose. Electron microscopic studies using horseradish peroxidase as a tracer revealed that elastase interrupts intercellular tight junctions of the epithelial lining, thereby increasing the permeability to macromolecules. The amounts of elastase used in this report did not cause interstitial or alveolar edema, as determined by both postmortem extravascular lung water volume measurement and morphological examination. The data indicate that the elastase is a potentially important virulence factor in...Continue Reading

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Citations

Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·R WilsonA D Jackson
Oct 7, 2003·American Journal of Respiratory Cell and Molecular Biology·Christelle CorauxEdith Puchelle
Aug 18, 2010·Antimicrobial Agents and Chemotherapy·Nobumasa AokiKeizo Yamaguchi
Jul 16, 2011·American Journal of Respiratory Cell and Molecular Biology·Curtis A ClarkAli O Azghani
Mar 1, 1993·Experimental Lung Research·J C Connelly, B T Peterson
Jun 10, 2009·Infection and Immunity·Irania AlarconSuzanne M J Fleiszig
Jan 5, 2014·Microbiology·Ali O AzghaniUsha R Pendurthi
Feb 1, 1992·Molecular Microbiology·M J Brumlik, D G Storey
Jun 1, 1992·American Journal of Respiratory Cell and Molecular Biology·A O AzghaniA R Johnson
Mar 16, 2000·Microbes and Infection·S Miyoshi, S Shinoda
Nov 28, 2002·Seminars in Perinatology·Marc D Foca
Apr 25, 2006·Médecine et maladies infectieuses·R Le BerreB Guery

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