Effects of Psoralen as an Anti-tumor Agent in Human Breast Cancer MCF-7/ADR Cells

Biological & Pharmaceutical Bulletin
Xiaohong WangZhenlin Yang

Abstract

Psoralen is a major active component of Psoralea corylifolia. In the present study, we analyzed psoralen-induced changes in human breast cancer MCF-7/ADR cells and investigated the underlying mechanisms of the anticancer effect on MCF-7/ADR cells. We measured cell viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to evaluate the cytotoxicity and multidrug resistance (MDR) reversal activity of psoralen. The cell cycle distribution and apoptosis, accumulation and efflux of rhodamine123 (Rh123), and P-glycoprotein (P-gp) expression levels of MCF-7/ADR cells treated with psoralen were all detected by flow cytometry (FCM). We assessed P-gp ATPase activity by monitoring ATP consumption. We evaluated the activity of nuclear factor-kappaB (NF-κB) and the expression of E-cadherin, vimentin and α-smooth muscle actin (SMA) involved in regulating epithelial-mesenchymal transition (EMT). The results showed that psoralen inhibited the proliferation of MCF-7/ADR cells as shown by G0/G1 phase arrest rather than encouraging apoptosis. It was also observed that psoralen reversed MDR through inhibiting ATPase activity rather than reducing P-gp expression. Our results further showed that psoralen inhibited the m...Continue Reading

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Citations

Dec 3, 2016·Journal of Experimental & Clinical Cancer Research : CR·Xiaohong WangZhenlin Yang
Dec 28, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Yanyan XuYanjun Zhang
Sep 27, 2019·Journal of Biochemical and Molecular Toxicology·Stepan A KremisTatiana S Frolova
Oct 6, 2020·Frontiers in Pharmacology·Yali RenCheng Peng
Aug 13, 2020·International Journal of Molecular Sciences·Salman AhmedRaffaele Capasso
May 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aneta GrabarskaAndrzej Stepulak
Jan 8, 2022·Nutrition and Cancer·Asefeh Dahmardeh GhalehnoKhadijeh Jamialahmadi

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