PMID: 2494943Apr 1, 1989Paper

Effects of puromycin and hydroxynorvaline on net production and intracellular degradation of collagen in human fetal lung fibroblasts

Archives of Biochemistry and Biophysics
F A BarileR S Bienkowski

Abstract

Amino acid substitutions in collagen that impair folding of the triple helix result in significant increases in intracellular degradation of newly synthesized collagen. We have studied the effects of agents that cause other kinds of defects in collagen: hydroxynorvaline, a threonine analog that interferes with association of pro-alpha chains; and puromycin, an antibiotic that causes premature release of nascent polypeptides. cis-Hydroxyproline and cycloheximide, whose effects on collagen synthesis and degradation have already been studied and reported, were employed as reference compounds. Human fetal lung fibroblasts were used in these experiments. All the agents inhibited total protein production, and all except cycloheximide inhibited percentage collagen production. Intracellular collagen degradation was increased in cultures exposed to puromycin, hydroxynorvaline, and cis-hydroxyproline, but not in cultures exposed to cycloheximide. These results suggest that pro-alpha chains that were either unassociated (due to hydroxynorvaline) or shortened (due to puromycin) were recognized as abnormal and degraded to the same extent as chains that contained cis-hydroxyproline. However, the increases in degradation could not account com...Continue Reading

References

Sep 1, 1988·Archives of Biochemistry and Biophysics·F A BarileR S Bienkowski
May 1, 1981·The Journal of Clinical Investigation·R A BergR G Crystal
Oct 1, 1984·Collagen and Related Research·R S Bienkowski
Aug 15, 1981·The Biochemical Journal·J N HildebranR B Low
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R A BergR G Crystal
Sep 15, 1981·Analytical Biochemistry·R S Bienkowski, C J Engels

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Citations

Jun 1, 1994·Cell Biology and Toxicology·F A BarileU Kristen
Feb 15, 1992·Experientia·P O Seglen, P Bohley
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Y BarnesF A Barile
Mar 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F A BarileD Hopkinson
Oct 13, 1998·In Vitro Cellular & Developmental Biology. Animal·F A Barile, M Cardona
Jan 1, 1990·Archives of Biochemistry and Biophysics·F A BarileR S Bienkowski
Sep 15, 2017·Physiological Reports·Bram PiersmaRuud A Bank
Nov 5, 1997·Experimental Cell Research·C R Ripley, R S Bienkowski
Apr 30, 1990·Biochemical and Biophysical Research Communications·R S BienkowskiB Steinmann

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