PMID: 11903975Mar 21, 2002Paper

Effects of putrescine accumulation in tobacco transgenic plants with different expression levels of oat arginine decarboxylase

Physiologia Plantarum
Mireia PanicotT Altabella

Abstract

Arginine decarboxylase (ADC; EC 4.1.1.19) is a key enzyme in one of the two possible ways to synthesize putrescine (Put) in plants. In previous work (Masgrau et al. 1997), we observed an altered phenotype (growth inhibition, leaf chlorosis and necrosis) in tobacco transgenic plants (Nicotiana tabacum L. var. Wisconsin-38) containing the oat ADC cDNA under the control of a tetracycline inducible promoter, the severity of which was correlated with Put content. Now we have analysed the T2 generation of a selected transgenic line (line 52), which in previous generations was characterized by presenting a moderate increase in ADC activity and polyamine levels, but no phenotype alterations. Studying two selected individuals, one with a high expression level of the transgene and the other with a moderate expression level, we demonstrate that only the one with increased polyamine content displays the altered (toxic) phenotype. The possible causes of toxicity have been analysed. The results suggest that either Put or its oxidation products, via diamine oxidase (DAO; EC 1.4.3.6), are the responsible factors for the deleterious effects observed in the transgenic plants.

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L A Guarino, S S Cohen
Mar 1, 1991·Archives of Biochemistry and Biophysics·R H Davis, J L Ristow
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·J I SchroederW Gassmann
May 1, 1994·The Plant Cell·N HibiY Yamada
Mar 1, 1997·The Plant Journal : for Cell and Molecular Biology·C MasgrauA F Tiburcio
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·M B WatsonR L Malmberg
May 24, 2000·Neurochemical Research·N Seiler
Sep 13, 1952·Nature·F J RICHARDS, R G COLEMAN
Nov 1, 1961·Archives of Biochemistry and Biophysics·T OKUYAMA, Y KOBAYASHI

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Citations

Jul 1, 2008·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Juan ZhaoQiuhong Yuan
Jul 28, 2005·The Plant Journal : for Cell and Molecular Biology·Rubén AlcázarTeresa Altabella
Aug 22, 2006·Journal of Plant Physiology·Xue WangJinzhao Hu
Sep 9, 2005·Journal of Plant Physiology·Juan J Camacho-CristóbalAgustín Gonzláez-Fontes
Jul 13, 2004·Plant Physiology·Akihiro ImaiTaku Takahashi

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