Effects of quercetin on the heat-induced cytotoxicity of prostate cancer cells

International Journal of Urology : Official Journal of the Japanese Urological Association
T NakanomaN Deguchi

Abstract

It has been demonstrated that prostate cancer cells are relatively sensitive to heat stress. We have reported that heat treatment at 43 degrees C increases the expression of heat shock protein 70 (hsp70) in prostate cancer cells, leading to apoptosis. Hsp70 is a protein that protects cells against heat damage. Cells with lower levels of hsp70 have been shown to have a higher sensitivity to heat stress. Therefore, downregulation of hsp70 is expected to enhance heat-induced inhibitory effects on cell growth. Quercetin has been reported to be an agent that inhibits hsp70 expression. The present study was undertaken to investigate the effects of quercetin and/or heat on the growth of prostate cancer cells in vitro. Three human prostate cancer cell lines were used: Lncap; PC-3; and JCA-1. The cells were treated with quercetin and/or heat. Alterations in the cell cycle and hsp70 expression were examined by means of flow cytometry (FCM). The apoptotic cells were detected by FCM using fluorescein isothiocyanate (FITC) labeled annexin V. Treatment with quercetin alone resulted in an apparent decrease of hsp70-positive cells and an increase of subG1 cells in JCA-1 and LNcap cells. Quercetin inhibited an increase of hsp70 expression after...Continue Reading

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Citations

Dec 8, 2010·Assay and Drug Development Technologies·James H AhnGabriela Chiosis
Aug 26, 2014·The Journal of Surgical Research·Anna M LeungThomas A Miller
Jan 1, 2014·Nutrition and Cancer·Katrin Sak
Oct 28, 2017·Future Medicinal Chemistry·Domenico IacopettaMaria Stefania Sinicropi
Jan 13, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Karol DokladnyThomas Y Ma
Jan 6, 2011·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Emel SahinSaadet Gümüslü
Mar 15, 2003·The Journal of Biological Chemistry·Elena A KomarovaAndrei V Gudkov
Jul 28, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardAbbas Basiri

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis