Abstract
Radiolabeled sucrose is often used to assess blood-brain barrier (BBB) injury in the rat, but published transfer constants (K[i]s) for sucrose permeation of the intact BBB (control K[i]s) are highly discrepant. A potential problem with the commonly used tracer, [14C(U)]sucrose, is radiolytic generation, preuse, of radiocontaminants that might readily penetrate the BBB. How such contaminants might affect measurements of sucrose K(i)s was examined for both the intact and the ischemically injured BBB. Three stocks of [14C(U)]sucrose were studied: newly purchased ("new"), 4-year-old, and 7-year-old. A high purity (99.9%) "new" and a 2-year-old stock of [3H(fructose-1)]sucrose were also tested. Pentobarbital-anesthetized male Sprague-Dawley rats were injected i.v. with each tracer separately (six to eight rats) and K(i)s in five brain regions were measured by the multiple-time graphical method. The "new" 14C-, "new" 3H-, and 2-year-old 3H-sucrose yielded comparable K(i)s , ranging from 1.2 +/- 0.1 to 2.4 +/- 0.3 nl x g(-1) x s(-1) (mean +/- SE) across the regions. The two old stocks of 14C-sucrose yielded significantly higher regional K(i)s : 5.1-6.3 (4-year-old) and 8.4-9.7 (7-year-old). Thin-layer chromatography of the three 14C-t...Continue Reading
References
Jul 21, 1978·Brain Research·S I RapoportK D Pettigrew
Jan 1, 1990·Neuroscience·M RavnborgM Blinkenberg
Oct 11, 1988·Brain Research·Q R SmithS I Rapoport
Jan 1, 1986·Journal of Neuroscience Research·E Preston, N Haas
Jul 9, 1986·Brain Research·Y S KimH M Wisniewski
Dec 1, 1984·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A H LockwoodF Napoleon
Jan 1, 1984·Acta Neuropathologica·M L SmithB K Siesjö
May 1, 1984·Brain Research·W M WilliamsS M Michaelson
May 1, 1984·Brain Research·W M WilliamsS M Michaelson
May 1, 1984·Brain Research·W M WilliamsS M Michaelson
Mar 1, 1983·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·C S PatlakJ D Fenstermacher
Apr 1, 1981·Journal of Neurochemistry·A Gjedde
Dec 23, 1981·Neuroscience Letters·B G Cragg, S C Phillips
Apr 1, 1980·Acta Physiologica Scandinavica·A GjeddeE Siemkowicz
Sep 1, 1993·NMR in Biomedicine·E Preston, D O Foster
Jan 4, 1993·Neuroscience Letters·E PrestonA Finsten
Mar 5, 1993·Neuroscience Letters·Y Z ZiylanJ M Bourre
May 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·L WeiJ Fenstermacher
Citations
Feb 24, 2001·Journal of Neurotrauma·E PrestonD Small
Feb 27, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mohammad K MiahReza Mehvar
May 10, 2005·Biophysical Journal·Gertjan VeldhuisRuud M Scheek
Aug 18, 2018·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Faleh AlqahtaniUlrich Bickel
Nov 20, 2020·Fluids and Barriers of the CNS·Hossam KadryLuca Cucullo
Jan 14, 2021·Journal of Labelled Compounds & Radiopharmaceuticals·Jeremy R Wolf