Effects of radiologic contrast media on human endothelial and kidney cell lines: intracellular pH and cytotoxicity

Academic Radiology
A Dascalu, Amir Peer

Abstract

Radiologic contrast media (CM) are hyperosmotic compounds injected undiluted into a patient's blood, in which they contact endothelial cells. For some types of cultured cells, the application of a hyperosmotic stimulus may cause intracellular pH (pHi) acidification that is related to the extent of hyperosmolality and that ultimately influences cellular function. Accordingly, endothelial and kidney cells, two types of cells known to be exposed to CM effects, were treated at relevant iodine concentrations with various CM (320-1500 mOsm) to determine whether cell exposure to CM can disturb the pH(i) and to examine the contribution of CM to cellular cytotoxicity. Ionic (n = 3) and nonionic (n = 3) CM were compared. Changes in the pH(i) of human vascular endothelial and kidney cell lines were monitored by use of a pH-sensitive fluorescent dye (2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester). The viability of cells treated with CM was determined by measuring the reduction of a tetrazolium salt (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenlytetrazolium bromide) to violet formazan, a reaction that requires the activity of mitochondrial dehydrogenase; this measurement was made with a microplate reader. The pH(i) of endot...Continue Reading

References

May 11, 1992·Transplantation·W A KillingerG Johnson
Aug 1, 1981·AJR. American Journal of Roentgenology·F A Burgener, D J Hamlin

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Citations

Aug 7, 2012·Investigative Radiology·Diana C LenhardGregor Jost
Feb 26, 2011·Clinical and Experimental Pharmacology & Physiology·Mauricio M Sendeski
Apr 20, 2014·BioMed Research International·Ashour MichaelMichele Andreucci
Jul 27, 2000·The British Journal of Radiology·M BardelliR Carretta
Oct 22, 2013·Yonsei Medical Journal·Seun Deuk HwangSoo Young Yoon
Jan 3, 2001·American Journal of Physiology. Renal Physiology·K HardiekC Deitrick

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