Effects of rapid cooling on articular cartilage

Cryobiology
J GuanZ F Cui

Abstract

In order to improve the technique and protocols of cryopreservation of articular cartilage, a study was carried out to assess the effects of rapid cooling on the intact articular cartilage. Cartilage slices with a thickness ranging from 0.2 to 0.5 mm taken from bovine metacarpal-phalangeal joints were subjected to rapid cooling by immersing them in liquid nitrogen with and without treatment of the VS55 cryoprotective agent (CPA). The ultrastructure, chondrocyte viability, swelling property, and glycosaminoglycan (GAG) content were then examined before and after cryopreservation to give qualitative and quantitative evaluation on the functional state of both chondrocytes and extracellular matrix. The transmission electron microscopy study demonstrated that damage to chondrocytes without CPA was far more pronounced than those with VS55 protection while the structure of the extracellular matrix altered little in either group. The cell viability assay showed that although the exposure to VS55 led to about 36% chondrocytes losing membrane integrity, the VS55 could provide protection to chondrocytes during rapid cooling and thawing, with approximately 51% of the cells having survived rapid cooling compared to fewer than 5% in the abse...Continue Reading

References

Jan 1, 1990·International Orthopaedics·N Kawabe, M Yoshinao
Jan 1, 1993·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K TavakolN S Schachar
Oct 1, 1994·British Journal of Rheumatology·J P Urban
May 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C OhlendorfH J Mankin
Sep 18, 1999·Osteoarthritis and Cartilage·V ManolopoulosP J Doherty
Mar 4, 2000·Transplantation Proceedings·K G BrockbankM J Taylor
Sep 7, 2002·Fertility and Sterility·Lilia L Kuleshova, Alex Lopata
Dec 11, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·N M JomhaL E McGann
Jun 9, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Madhura D GoleIsrael Ziv
Jul 19, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·James M WilliamsBrian J Cole

❮ Previous
Next ❯

Citations

Dec 7, 2011·Connective Tissue Research·Jennifer Jane BaraSally Roberts
Dec 25, 2012·Colloids and Surfaces. B, Biointerfaces·Shaw-Ruey LyuWen-Hsin Hsieh
Jun 16, 2012·Biomaterials·Nadr M JomhaLocksley E McGann
Jun 5, 2012·Cryobiology·Issei OnariHiroyuki Tsuchiya
Oct 14, 2010·Cryobiology·Nadr M JomhaLocksley E McGann
Aug 8, 2009·The Journal of Thoracic and Cardiovascular Surgery·Hongcan ShiJun Wu
Dec 2, 2008·Cryobiology·Nadr M JomhaLocksley E McGann
Nov 14, 2014·Cell Biochemistry and Biophysics·Zenon PawlakWojciech Hagner
Oct 25, 2011·Cryobiology·Gaye Cetinkaya, Sezen Arat

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
K TavakolN S Schachar
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Nadr M JomhaL E McGann
© 2022 Meta ULC. All rights reserved