Effects of reaction conditions on RNA secondary structure and on the helicase activity of Escherichia coli transcription termination factor Rho

Journal of Molecular Biology
K M WalstromP H von Hippel

Abstract

The ATPase and helicase activities of the Escherichia coli transcription termination protein rho have been studied under a variety of reaction conditions that alter its transcription termination activity. These conditions include KCl, KOAc, or KGlu concentrations from 50 to 150 mM and Mg(OAc)2 concentrations from 1 to 5 mM (in the presence of 1 mM ATP). In higher KCl or higher Mg(OAc)2 concentrations we found that the translocation of rho hexamers along RNA was slower and less processive than the same process measured at 50 mM monovalent salt concentrations and 1 mM Mg(OAc)2. The ATPase activity of rho was also decreased under reaction conditions that slowed translocation. RNA melting experiments showed that the decreased ATPase activity of rho and the slower helicase activity at increased KCl or Mg(OAc)2 concentrations are accompanied by a concomitant increase in the secondary structure of the RNA portion of the helicase substate. In contrast, the ATPase activity of rho in the presence of poly(rC), a synthetic RNA that does not form salt-concentration-dependent secondary structure, was shown to be the same in each of the three monovalent salts. Thus, the salts do not directly affect the structure or conformation of the rho pro...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·W J O'Sullivan, G W Smithers
Jul 1, 1992·Protein Science : a Publication of the Protein Society·J GeiselmannP H von Hippel
Jul 1, 1992·Protein Science : a Publication of the Protein Society·J Geiselmann, P H von Hippel
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D J JinC A Gross
May 24, 1985·Nucleic Acids Research·W D MorganP H von Hippel
Jul 5, 1989·Journal of Molecular Biology·E C Ruteshouser, J P Richardson
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·X J Yang, J W Roberts
Sep 1, 1988·Trends in Biochemical Sciences·D G Bear, D S Peabody
Jan 1, 1986·Annual Review of Biochemistry·T Platt
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C Lowery-Goldhammer, J P Richardson
Apr 15, 1980·Journal of Molecular Biology·G R Galluppi, J P Richardson
Mar 25, 1982·Journal of Molecular Biology·L R Finger, J P Richardson
Mar 1, 1994·Molecular Microbiology·T Platt
Jan 19, 1996·The Journal of Biological Chemistry·J P Richardson
Jun 10, 1997·Biochemistry·D P Mascotti, T M Lohman
May 20, 1998·Nature Structural Biology·T J AllisonG S Rule

❮ Previous
Next ❯

Citations

Feb 11, 2015·Nucleic Acids Research·Veronika GochevaMarcelo Nollmann
Aug 29, 2007·The Journal of Biological Chemistry·Annie SchwartzMarc Boudvillain
Dec 9, 2008·Journal of Molecular Biology·Maarten H de SmitCornelis W A Pleij

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.