PMID: 11918852Mar 29, 2002Paper

Effects of reactive oxygen species on lymphokine-activated killer cells in patients with bladder cancer

Acta Pharmacologica Sinica
Zhi-Ping WangJia-Ji Wang

Abstract

To investigate the effect of reactive oxygen species on the proliferation of lymphokine-activated killer (LAK) cells in patients with bladder cancer and their cytolysis to bladder tumor cells. Sodium nitroprusside (SNP) was used as nitric oxide (NO) donor. The superoxide anion (O2-.) was generated in the complete medium (CM) supplemented with N-methylphenazonium methyl sulfate (PMS) 3-120 micromol/L and nicotinamide adenine dinucleotide (NADH) 18 - 600 micromol/L. The hydroxyl radical (.OH) was produced by adding ascorbic acid (AA) 0.5 - 400 micromol/L and ferrous sulfate (Fe2+) 0.05 - 40 micromol/L in CM. LAK cell proliferation and cytotoxicity were assayed in the presence of NO, .OH, or O2-. Bladder cancer cell lines BIU-87 and EJ were cultured as target cells and cytotoxicity of LAK cells were determined by MTT assay. The proliferation of LAK cells induced by interleukin-2 (IL-2) was inhibited by hydroxyl radical from 48 h to 96 h in a dose-dependent fashion and was inhibited to 34.5 % compared with control at 96 h in the concentration of ascorbic acid 400 micromol/L and ferrous sulfate 40 micromol/L. The inhibition induced by.OH can be overcome by certain concentrations of mannitol or editic acid. On the contrary, the proli...Continue Reading

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