Effects of reaggregated granulosa cells and oocytes derived from early antral follicles on the properties of oocytes grown in vitro

The Journal of Reproduction and Development
Ayano OiH Iwata

Abstract

In this study, we examined the effects of reconstructed oocyte-granulosa cell complexes (OGCs) on the development of porcine oocytes derived from early antral follicles (EAFs; 0.5-0.7 mm in diameter). When denuded oocytes were cocultured with granulosa cells derived from other EAFs, the oocytes and granulosa cells aggregated to form OGCs after 2 days of culture. After 14 days of culture, we compared cell number, oocyte diameter, and oocyte chromatin configuration in unmanipulated (natural) OGCs, reconstructed OGCs, and OGCs collected from antral follicles (AFs, 3.0-6.0 mm in diameter). The diameters of oocytes from reconstructed OGCs grown in vitro were not different from those of oocytes from natural OGCs, although they were significantly smaller than those of oocytes from antral follicle (AF) OGCs. Oocyte chromatin configuration did not differ among the 3 OGC groups, but the oocyte nuclear maturation rate was lower in the reconstructed OGCs and higher in the AF OGCs. However, when the in vitro culture period for the reconstructed OGCs was extended by 2 days, the nuclear maturation rate of oocytes from reconstructed OGCs was similar to that of oocytes from natural OGCs. In addition, blastocysts were successfully obtained from ...Continue Reading

References

Dec 26, 2001·Biology of Reproduction·Koji YoshiokaShokichi Iwamura
May 13, 2004·Journal of Cell Science·Gregory I VeitchGerald M Kidder
Aug 5, 2004·Molecular Reproduction and Development·Xing-Shen SunJing-He Tan
Aug 5, 2004·Angewandte Chemie·Eric J FechterPeter B Dervan
Jul 11, 2009·Journal of Ovarian Research·Makoto OrisakaFumikazu Kotsuji
Dec 17, 2009·Reproduction, Fertility, and Development·Mario Binelli, Bruce D Murphy
May 4, 2010·Molecular Human Reproduction·Zhongwei Huang, Dagan Wells
Nov 7, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·M EndoH Iwata
Apr 9, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Anne-Laure NivetMarc-André Sirard

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Citations

Nov 20, 2016·Molecular Reproduction and Development·Yasuhisa MunakataHisataka Iwata
Mar 1, 2017·Annals of Biomedical Engineering·Kanako MorohakuYayoi Obata
Oct 8, 2019·The Journal of Reproduction and Development·Yasuhisa MunakataHisataka Iwata
Mar 4, 2020·Zygote : the Biology of Gametes and Early Embryos·Tania Janeth Porras-Gómez, Norma Moreno-Mendoza
May 16, 2019·Reproduction in Domestic Animals = Zuchthygiene·Nathalie RaffelLaura Lotz
Jul 18, 2019·Reproductive Medicine and Biology·Hidenori ShibaharaHisataka Iwata
May 13, 2017·The Journal of Reproduction and Development·Hisataka Iwata
Aug 24, 2021·The Journal of Reproduction and Development·Mihoko FushiiTakashi Miyano

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Methods Mentioned

BETA
FCS

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