PMID: 8971290Dec 1, 1996Paper

Effects of rebamipide on bile acid-induced inhibition of gastric epithelial repair in a rabbit cell culture model

Alimentary Pharmacology & Therapeutics
S WatanabeN Sato

Abstract

Anti-ulcer agents exert various functional effects on gastric epithelial cells. The effects of a novel gastro-cytoprotective agent (rebamipide) on epithelial restoration following bile acid damage were assessed using primary cultured rabbit gastric epithelial cells. Rebamipide was added to complete confluent cell sheets with deoxycholic acid just after creating a cell-free wound (2 mm2). The restoration was monitored and analysed by phase contrast microscopy and an image analyser for 48 h. The migration speed was measured during the initial 3 h after wounding. Cell proliferation was detected by staining for bromodeoxyuridine (BrdU) at 12-h intervals. The labelling index was calculated per unit area. The major cytoskeletal protein actin was detected by immunohistochemical staining. In the controls, restoration was completed 48 h following wounding. Deoxycholic acid retarded this process. The addition of rebamipide to deoxycholic acid abolished the bile acid-induced retardation. The migration speed was 26 microns/h in the controls. 15 microns/h in the deoxycholic acid group and 27 microns/h in the deoxycholic acid plus rebamipide group. In the controls, BrdU-positive cells, which were rarely detected in the initial 24 h, were max...Continue Reading

Citations

Jan 12, 2007·Digestive Diseases and Sciences·Tamotsu MatsuhashiSumio Watanabe
Jan 6, 2001·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Y ArakiT Bamba
Feb 21, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Hidekazu SuzukiHiromasa Ishii
Feb 14, 2007·Journal of Gastroenterology and Hepatology·Ryuichi FurutaHidemi Goto
Aug 20, 2003·Alimentary Pharmacology & Therapeutics·T JohM Itoh
Feb 2, 2013·Digestive Endoscopy : Official Journal of the Japan Gastroenterological Endoscopy Society·Takashi KawaiShinya Goto

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