Effects of recombinant precursor pathway variations on poly[(R)-3-hydroxybutyrate] synthesis in Saccharomyces cerevisiae

Journal of Biotechnology
Ross Carlson, Friedrich Srienc

Abstract

Different recombinant R-3-hydroxybutyryl-CoA (3-HB) synthesis pathways strongly influenced the rate and accumulation of the biopolymer poly[(R)-3-hydroxybutyrate] (PHB) in Saccharomyces cerevisiae. It has been previously shown that expression of the Cupriavidus necator PHB synthase gene leads to PHB accumulation in S. cerevisiae [Leaf, T., Peterson, M., Stoup, S., Somers, D., Srienc, F., 1996. Saccharomyces cerevisiae expressing bacterial polyhydroxybutyrate synthase produces poly-3-hydroxybutyrate. Microbiology 142, 1169-1180]. This finding indicates that native S. cerevisiae expresses genes capable of synthesizing the correct stereochemical substrate for the synthase enzyme. The effects of variations of 3-HB precursor pathways on PHB accumulation were investigated by expressing combinations of C. necator PHB pathway genes. When only the PHB synthase gene was expressed, the cells accumulated biopolymer to approximately 0.2% of their cell dry weight. When the PHB synthase and reductase gene were co-expressed, the PHB levels increased approximately 18 fold to about 3.5% of the cell dry weight. When the beta-ketothiolase, reductase and synthase genes were all expressed, the strain accumulated PHB to approximately 9% of the cell d...Continue Reading

References

Aug 5, 1976·Nature·J L Spudich, D E Koshland
Jan 2, 1992·Gene·T W ChristiansonP Hieter
Jan 1, 1991·Methods in Enzymology·F Sherman
Jan 1, 1990·Methods in Enzymology·A B Rose, J R Broach
Jan 1, 1973·Advances in Microbial Physiology·E A Dawes, P J Senior
Jul 1, 1982·Applied and Environmental Microbiology·A G Ostle, J G Holt
Feb 11, 1983·Science·R A HitzemanD V Goeddel
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L GuarenteP Gifford
Apr 1, 1993·Genes & Development·M BraunsteinJ R Broach
Jul 1, 1996·Applied and Environmental Microbiology·M D WilliamsD H Sherman
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·H H McAdams, A Arkin
Aug 1, 1997·International Journal for Parasitology·J Walker, J Barrett
Apr 1, 1999·Biotechnology and Bioengineering·T A Leaf, F Srienc
Jul 23, 1999·International Journal of Biological Macromolecules·A S Kelley, F Srienc
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·M E John, G Keller
Mar 14, 2001·Journal of Biotechnology·B Kessler, B Witholt
Mar 12, 2002·FEMS Microbiology Letters·Yves PoirierJean MacDonald-Comber Petétot
Jul 13, 2002·Biotechnology and Bioengineering·Ross CarlsonFriedrich Srienc
Aug 17, 2002·Science·Michael B ElowitzPeter S Swain
Dec 5, 2003·Applied Microbiology and Biotechnology·Y TerentievG Kunze
Nov 17, 2004·Journal of Experimental Botany·David A Fell
Mar 15, 2005·Biomacromolecules·Natarajan VijayasankaranFriedrich Srienc
Dec 13, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·James KacmarFriedrich Srienc
Jan 5, 2006·Applied and Environmental Microbiology·Bo ZhangFriedrich Srienc
Jan 1, 1999·Bioscience, Biotechnology, and Biochemistry·H NakashitaI Yamaguchi

❮ Previous
Next ❯

Citations

Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·Elke Nevoigt
Jan 29, 2011·Journal of Biomedicine & Biotechnology·Xueyang FengYinjie J Tang
Jun 22, 2011·Microbial Cell Factories·Arul M VarmanYinjie J Tang
Aug 31, 2014·Biotechnology Advances·Quanfeng Liang, Qingsheng Qi
Mar 5, 2013·Biotechnology and Bioengineering·Kanokarn KocharinJens Nielsen
Apr 9, 2015·AMB Express·Anders G SandströmMarie F Gorwa-Grauslund
Dec 2, 2011·FEMS Yeast Research·Nancy A Da Silva, Sneha Srikrishnan
Nov 9, 2016·Journal of Industrial Microbiology & Biotechnology·Zheng-Jun LiGregory Stephanopoulos
Feb 27, 2008·Chemistry, an Asian Journal·Yessi PermanaKyoko Nozaki
Dec 17, 2018·Biotechnology Advances·Suryang KwakJin-Ho Seo
Apr 27, 2021·Journal of Industrial Microbiology & Biotechnology·Anna YlinenMervi Toivari
Jun 21, 2021·Bioresource Technology·Bhoomika YadavPatrick Drogui

❮ Previous
Next ❯

Related Concepts

Related Feeds

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.