PMID: 9167937Apr 1, 1997Paper

Effects of respiratory burst inhibitors on nitric oxide production by human neutrophils

Free Radical Research
M C CarrerasJ J Poderoso

Abstract

Human neutrophils (PMN) activated by N-formylmethionyl-leucyl-phenylalanine (fMLP) simultaneously release nitric oxide (.NO), superoxide anion (O2.-) and its dismutation product, hydrogen peroxide (H2O2). To assess whether .NO production shares common steps with the activation of the NADPH oxidase, PMN were treated with inhibitors and antagonists of intracellular signaling pathways and subsequently stimulated either with fMLP or with a phorbol ester (PMA). The G-protein inhibitor, pertussis toxin (1-10 micrograms/ml) decreased H2O2 yield without significantly changing .NO production in fMLP-stimulated neutrophils; no effects were observed in PMA-activated cells. The inhibition of tyrosine kinases by genistein (1-25 micrograms/ml) completely abolished H2O2 release by fMLP-activated neutrophils; conversely, .NO production increased about 1.5- and 3-fold with fMLP and PMA, respectively. Accordingly, orthovanadate, an inhibitor of phosphotyrosine phosphatase, markedly decreased .NO production and increased O2.- release. On the other hand, inhibition of protein kinase C with staurosporine and the use of burst antagonists like adenosine, cholera toxin or dibutyryl-cAMP diminished both H2O2 and .NO production. The results suggest that...Continue Reading

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Citations

Jul 30, 1999·Journal of the Neurological Sciences·E M GattoJ J Poderoso
Dec 8, 2000·Thrombosis Research·S Sethi, M Dikshit
Oct 24, 2003·Cell Calcium·Marguerite R KelherChristopher C Silliman
Aug 9, 2001·Antioxidants & Redox Signaling·L B Valdez, A Boveris
Apr 1, 2010·Toxins·Susana CubillosKatja Lehmann
Sep 19, 2006·European Journal of Pharmacology·Stanislava StanojevićMirjana Dimitrijević
Jul 5, 2016·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Michaela PilchElisabeth Aberer
Nov 23, 2017·International Journal of Molecular Sciences·Marcin Lewandowski, Krzysztof Gwozdzinski

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