Effects of retinoic acid (all-trans and 9-cis) on tumor progression in small-cell lung carcinoma

Biochemical and Biophysical Research Communications
M GüzeyH F DeLuca

Abstract

The two stereoisomers of retinoic acid (RA), all-trans and 9-cis-RA, are regulators of cell proliferation, differentiation and apoptosis. In this study, the aim was to evaluate the effects of all-trans-and 9-cis-RA on cell growth, proliferation, and on the induction of apoptosis in the human small cell lung carcinoma (SCLC) cell lines NCI-H82 and NCI-H209. The application of various concentrations of all-trans and 9-cis-RA were able to inhibit cell growth and proliferation. Moreover, 3H-thymidine incorporation was inhibited and the number of viable cells decreased, suggesting that all-trans-RA and 9-cis-RA can inhibit cell proliferation in a dose dependent manner. Morphological examinations (light, electron and fluorescence microscopy) demonstrated that both retinoids had profound effects on the induction of apoptosis. Our investigation also showed that, compared to all-trans-RA, 9-cis-RA is a stronger inducer for the inhibition of cell growth and proliferation and that it is more effective in the induction of apoptosis in small cell lung carcinoma cells in culture.

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Citations

Jun 13, 2000·Cancer Biotherapy & Radiopharmaceuticals·A M BerghellaD Adorno
Jan 15, 2003·Human Reproduction·Fu-Jen HuangYan-Der Hsuuw
May 1, 2007·Cellular & Molecular Biology Letters·Wenwen LiuAnli Jiang
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Mar 25, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ji-Youn HanHo-Young Lee

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis