Effects of retinol on the in vitro development of Bos indicus embryos to blastocysts in two different culture systems

Reproduction in Domestic Animals = Zuchthygiene
P F LimaM H B Santos

Abstract

The objective of this study was to evaluate the effect of retinol on the in vitro development of early embryos of cultured Bos indicus (Expt 1) to the blastocyst stage in medium simplex of optimization (KSOM) or sintetic fluid of oviduct (SOF) or co-cultured (Expt 2) with an oviduct cell monolayer (OCM) in KSOM or SOF. A total of 3149 cumulus-oocyte complexes obtained by aspirating follicles (2-5 mm diameter) from ovaries of slaughtered animals were selected for IVM and incubated in TCM 199 supplemented with 25 mM HEPES at 39 degrees C in air with 5% CO(2) and maximum humidity for 24 h. In vitro fertilization (IVF) was performed in modified defined medium (mDM) medium. Eighteen hours after IVF, cumulus cells were removed and presumptive zygotes were randomly allocated to the experimental groups. Zygotes cultured (Expt 1) in KSOM + retinol, KSOM, SOF + retinol and SOF were incubated in maximum humidity at 39 degrees C, 5% CO(2), 5% O(2) and 90% N(2). Zygotes co-cultured (Expt 2) in KSOM + retinol + OCM, KSOM + OCM, SOF + retinol + OCM and SOF + OCM were incubated at 39 degrees C, 5% CO(2). In both experiments media were partially changed 48 h after IVF and unfertilized ova were removed. Afterwards embryos were kept in culture or...Continue Reading

References

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Citations

Jul 13, 2012·Zygote : the Biology of Gametes and Early Embryos·M J Maya-SorianoM López-Béjar
Nov 23, 2006·Theriogenology·Nicolas M Orsi, Judith B Reischl
Feb 27, 2013·Acta Veterinaria Hungarica·Adauto ChiamentiMarcos Antonio Lemos Oliveira
Aug 24, 2013·Zygote : the Biology of Gametes and Early Embryos·M J Maya-SorianoM Lopez-Bejar
Nov 3, 2016·Zygote : the Biology of Gametes and Early Embryos·Juliana C Z ConceiçãoMarcos A L Oliveira
Oct 6, 2018·Zygote : the Biology of Gametes and Early Embryos·Ahmed M ElomdaGamal M K Mehaisen
Oct 1, 2009·Reproduction in Domestic Animals = Zuchthygiene·A ChiamentiM A L Oliveira
Jun 24, 2020·Tropical Animal Health and Production·Pamela Ramos-DeusMarcos Antonio Lemos Oliveira

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