Effects of salicylate on voltage-gated sodium channels in rat inferior colliculus neurons

Hearing Research
Yanxing Liu, Xuepei Li

Abstract

To investigate the effects of the tinnitus inducer, sodium salicylate, on voltage-gated sodium channels, we studied freshly dissociated inferior colliculus neurons of rats by the whole-cell voltage clamp method. Salicylate blocked sodium channels in concentration-dependent manner (0.1-10 mM), and the IC50 value of salicylate was estimated to be 1.43 mM after application. The sodium conductance-voltage curve did not shift along the voltage axis with salicylate application. In contrast, the steady-state sodium channel inactivation curve was shifted by about 9 mV in the hyperpolarizing direction. In addition, salicylate delayed the sodium channel recovery from inactivation by increasing the slow time constant. It was concluded that salicylate bound to the resting and inactivated sodium channels to cause blocking, with a higher affinity for the latter state. Our results suggest that salicylate causes a concentration-dependent blockade of voltage-gated sodium channels and shifts the inactivation curve to more hyperpolarized potentials, which could be related to the mechanism of salicylate-induced tinnitus.

References

Oct 1, 1992·The Laryngoscope·A R MøllerM Yokota
Mar 1, 1988·The Laryngoscope·P J JastreboffC T Sasaki
Oct 1, 1986·Archives of Otolaryngology--head & Neck Surgery·P J JastreboffC T Sasaki
Apr 1, 1985·Clinical Otolaryngology and Allied Sciences·A W BlayneyB H Colman
Nov 1, 1965·Archives of Otolaryngology·E N Myers, J M Bernstein
Jul 8, 1996·Neuroreport·E Wallhäusser-FrankeG Langner
Oct 3, 1999·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·C A BauerM Wyder
Aug 30, 2000·Hearing Research·C A BauerD M Caspary
Apr 1, 1952·The Journal of Physiology·A L HODGKIN, A F HUXLEY

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Citations

Feb 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Luisa L ScottNace L Golding
Aug 8, 2009·Neuroscience Letters·Rosario VegaEnrique Soto
Jun 29, 2018·CNS & Neurological Disorders Drug Targets·You ZhouYong-Hua Ji
Sep 14, 2007·Acta Oto-laryngologica·Yiwen ZhengCynthia L Darlington
May 4, 2021·Naunyn-Schmiedeberg's Archives of Pharmacology·Soomaayeh HeysieattalabSamira Choopani

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