Effects of Salmonella enterica serovar Enteritidis on cellular recruitment and cytokine gene expression in caecum of vaccinated chickens

Vaccine
Bárbara González CarvajalAngela Berndt

Abstract

Although vaccination of poultry is a suitable method to limit human food borne gastroenteritis caused by Salmonella (S.), the immune mechanisms responsible for a longer lasting protection against Salmonella infection in birds are not completely understood. To reveal unique protection-related immune parameters, day-old chicks were vaccinated with a commercial live S. Enteritidis vaccine and challenged with wild-type S. Enteritidis 147N at day 56 of life. The bacterial cell count was determined in gut and liver, while the immune cell composition and cytokine gene expression patterns were analysed by immunohistochemistry and quantitative real-time RT-PCR in caecum samples. The presented data suggest that the vaccine-elicited immune protection against the Salmonella wild-type infection was rather related to the bacterial count in gut mucosa and liver than to the colonisation in gut lumen. The higher number of Salmonella wild-type organisms found in caecal wall and liver of the non-immunised compared to immunised birds after challenge correlated with a more pronounced gene expression rate for IL-8, LITAF, iNOS, IL-12 and IFN-gamma. In contrast, immunised birds exhibited higher amounts of CD8(+) T cells as well as IgA than the non-im...Continue Reading

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Citations

Dec 19, 2014·Avian Diseases·Soressa M KitessaRobert J Hughes
Apr 28, 2010·Infection and Immunity·Ilse D JacobsenMatthias Brock
Nov 11, 2015·Veterinary Immunology and Immunopathology·Maria BraukmannAngela Berndt
Aug 2, 2014·International Journal of Medical Microbiology : IJMM·Michael R KnittlerKonrad Sachse
Apr 25, 2017·Research in Veterinary Science·Martina HesseRita Weber
Mar 15, 2018·Avian Pathology : Journal of the W.V.P.A·Breno C B BeirãoLuiz Felipe Caron
Aug 11, 2020·Journal of Animal Physiology and Animal Nutrition·MiJin LeeJinSoo Kim
Apr 17, 2020·Frontiers in Immunology·Sravya SreekantapuramIlse D Jacobsen

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