Effects of sampling method on foliar δ (13)C of Leymus chinensis at different scales

Ecology and Evolution
Yanjie LiuHaishan Niu

Abstract

Stable carbon isotope composition (δ (13)C) usually shows a negative relationship with precipitation at a large scale. We hypothesized that sampling method affects foliar δ (13)C and its response pattern to precipitation. We selected 11 sites along a precipitation gradient in Inner Mongolia and collected leaves of Leymus chinensis with five or six replications repeatedly in each site from 2009 to 2011. Additionally, we collected leaves of L. chinensis separately from two types of grassland (grazed and fenced) in 2011. Foliar δ (13)C values of all samples were measured. We compared the patterns that foliar δ (13)C to precipitation among different years or different sample sizes, the differences of foliar δ (13)C between grazed and fenced grassland. Whether actual annual precipitation (AAP) or mean annual precipitation (MAP), it was strongly correlated with foliar δ (13)C every year. Significant difference was found between the slopes of foliar δ (13)C to AAP and MAP every year, among the slopes of foliar δ (13)C to AAP from 2009 to 2011. The more samples used at each site the lower and convergent P-values of the linear regression test between foliar δ (13)C and precipitation. Furthermore, there was significant lower foliar δ (13...Continue Reading

References

Mar 29, 2005·Evolution; International Journal of Organic Evolution·Neil J HausmannEllen L Simms
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Aaron F DiefendorfKatherine H Freeman
Dec 25, 2010·The New Phytologist·I Colin PrenticeGuohong Wang
Sep 1, 1988·Oecologia·James R Ehleringer, Tamsie A Cooper

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