Effects of sanguiin H-6, a component of Sanguisorbae Radix, on lipopolysaccharide-stimulated nitric oxide production

Biochemical Pharmacology
T YokozawaK Kitani

Abstract

The present study was conducted to evaluate the effect of sanguiin H-6, a component of Sanguisorbae Radix, on the production of nitric oxide (NO), using macrophages activated by lipopolysaccharide (LPS). Sanguiin H-6 inhibited nitrite production, taken as an index for NO, in a concentration-dependent fashion. This compound decreased inducible NO synthase (iNOS) activity, with the inhibitory effect at a concentration of 25 microM being equal to that of the known iNOS inhibitor aminoguanidine at 50 microM. However, unlike aminoguanidine, sanguiin H-6 was associated with improved cell viability. Reverse transcription-polymerase chain reaction analysis revealed that the expression of iNOS mRNA in activated macrophages was suppressed by sanguiin H-6 in a concentration-dependent manner. In addition, sanguiin H-6 even at a low concentration showed a clear scavenging effect on the NO generated from sodium nitroprusside (an NO donor). These findings indicate that not only does sanguiin H-6 act directly as an NO scavenger, but it also inhibits NO production in LPS-activated macrophages by the concomitant inhibition of iNOS mRNA induction and enzyme activity.

References

Nov 1, 1992·Archives of Biochemistry and Biophysics·H IschiropoulosJ S Beckman
May 29, 1992·Biochemical and Biophysical Research Communications·R A Pittner, J A Spitzer
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M W RadomskiS Moncada
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J Stuehr, M A Marletta
Feb 1, 1983·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·I Nishioka
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Oct 27, 1995·Journal of Medicinal Chemistry·J F KerwinP L Feldman
Nov 1, 1993·British Journal of Pharmacology·M J GriffithsT W Evans
Mar 1, 1994·The Biochemical Journal·R G Knowles, S Moncada
Jan 10, 1995·Archives of Biochemistry and Biophysics·D J Wolff, A Lubeskie
Jan 1, 1997·The Journal of Pharmacy and Pharmacology· Sreejayan, M N Rao
May 1, 1997·Proceedings of the Society for Experimental Biology and Medicine·S S LefkowitzD L Lefkowitz
Mar 15, 2000·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·T YokozawaT Tanaka
Apr 4, 2000·Biological & Pharmaceutical Bulletin·C P ChenK Kitani
Jun 23, 2000·Biological & Pharmaceutical Bulletin·T YokozawaK Kitani
Dec 1, 1964·Analytical Biochemistry·R F ITZHAKI, D M GILL

❮ Previous
Next ❯

Citations

Nov 5, 2008·Biological & Pharmaceutical Bulletin·Thi Thuy Ha NguyenYeon Hee Seong
Apr 2, 2009·Chemical & Pharmaceutical Bulletin·Hajime MaedaIsao Kouno
Aug 2, 2008·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Takashi Tanaka
Jan 5, 2016·Journal of Agricultural and Food Chemistry·Dahae LeeTakayuki Shibamoto
Sep 5, 2012·International Journal of Biological Macromolecules·Lin ZhangT Longvah
Feb 13, 2018·The American Journal of Chinese Medicine·Eungyeong JangJang-Hoon Lee
Jun 6, 2006·Medicinal Research Reviews·Ana OlmosSalvador Máñez
Feb 26, 2020·Chemistry & Biodiversity·Marek BunseDietmar Rolf Kammerer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.