Effects of saponins and glycoalkaloids on the permeability and viability of mammalian intestinal cells and on the integrity of tissue preparations in vitro

Toxicology in Vitro : an International Journal Published in Association with BIBRA
J M GeeA H Penninks

Abstract

The effects of potato and tomato glycoalkaloids and a saponin mixture from Gypsophila were investigated in cytotoxicity studies (neutral red uptake, mitochondrial MTT reduction and release of lactate dehydrogenase), using cultured cell lines of rat and human intestinal mucosal epithelium. Experiments to assess the effects of these compounds on the integrity of the intestinal epithelium were also carried out using preparations of isolated rat jejunum in vitro. By investigating the effect of these compounds on cultured cells and on intestinal tissue preparations, changes in membrane integrity, as evidenced by lactate dehydrogenase leakage in cell culture, could be confirmed in a system more relevant to the whole gut. Of the compounds tested, alpha-tomatine was consistently the most potent in all tests, and indications of a synergistic effect on membrane depolarization were observed between alpha-chaconine and alpha-solanine at total glycoalkaloid concentrations of less than 1 mM (< 0.86 mg/ml), with an optimum when the former comprised 25% of the mixture. An increase in the apparent permeability of the brush border was observed at sublethal concentrations of the compounds, and this may have important implications with respect to ...Continue Reading

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Citations

Jul 1, 1997·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J T BlankemeyerM Friedman
Aug 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A NaraiM Shimizu
Sep 15, 2011·The British Journal of Nutrition·Elvis M ChikwatiÅshild Krogdahl
Dec 21, 2002·The British Journal of Nutrition·George FrancisKlaus Becker
Dec 14, 2002·Inflammatory Bowel Diseases·Bijal PatelRichard N Fedorak
Dec 3, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Anne Marie BakkeÅshild Krogdahl
Jan 23, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Jéssica S Dos ReisFlávia M O B Saad
Oct 5, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Florian Herrmann, Michael Wink
Aug 17, 2011·Biotechnology Advances·Assunta PelosiAmanda M Walmsley
Jun 12, 2009·Journal of Agricultural and Food Chemistry·Mendel FriedmanNobuyuki Kozukue
Mar 12, 2011·Journal of Animal Physiology and Animal Nutrition·D CarlsonS-E Jacobsen
Mar 16, 2011·Journal of Agricultural and Food Chemistry·Sinead Eileen MilnerAnita Rose Maguire
Aug 14, 2020·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Bettina Grasl-Kraupp
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daniela PacificoCarlotta Balconi

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