Effects of scaffold/matrix alteration on centromeric function and gene expression.

The Journal of Biological Chemistry
Huseyin SumerK H Choo

Abstract

We have previously described a 3.5-Mb domain of enhance scaffold/matrix attachment region (S/MAR) at a human neocentromere, and normal expression of underlying genes within this region. We also reported that partial inhibition of histone deacetylation using 33 nmtrichostatin A (TSA) resulted in a shift in the position of the CENP-A-binding domain within the neocentromere, with no noticeable effects on mitotic segregation function. In this study, 33 nM TSA caused a reduction in the size of the enhanced S/MAR domain of one-half to 1.7 Mb. Treatment with a DNA-intercalating drug distamycin A (DST) at 75 microg/ml resulted in a size reduction of the enhanced S/MAR domain at the neocentromere of two-thirds to 1.2 Mb, and that of the CENP-A-binding domain of 40%, from 330 to 196 kb, with no significant shift in the position of the latter domain. Other DST effects include mitotic chromosomal missegregation, reduction in the levels of Topo IIalpha, CENP-A, CENP-C, and HP1alpha, and an increase in mitotic checkpoint protein BubR1. TSA or DST treatment similarly resulted in a significant reduction, by approximately 20 and 50%, respectively, in the size of the enhanced S/MAR domain at the alpha-satellite DNA of a native chromosome 10 cent...Continue Reading

References

Jan 1, 1979·Cytogenetics and Cell Genetics·G PranteraA Rocchi
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J P AbadA Villasante
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E W JabsG R Cutting
May 1, 1985·The Journal of Cell Biology·W C EarnshawL F Liu
Oct 23, 1974·Biochemical and Biophysical Research Communications·R Berezney, D S Coffey
Dec 15, 1983·Experientia·S Faccio Dolfini, A Bonifazio Razzini
Jan 1, 1984·The Journal of Cell Biology·W C EarnshawN Rothfield
Aug 1, 1996·Chromosoma·P L StrisselH Swift
Jan 20, 1998·Trends in Genetics : TIG·G H Karpen, R C Allshire
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·P KalitsisK H Choo
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·S A LelièvreM J Bissell
Feb 5, 1999·Human Molecular Genetics·A E BarryK H Choo
Mar 24, 1999·Mammalian Genome : Official Journal of the International Mammalian Genome Society·R SafferyK H Choo
Apr 8, 2000·Trends in Cell Biology·K H Choo
May 10, 2000·Current Opinion in Cell Biology·A L Pidoux, R C Allshire
Sep 14, 2000·Proceedings of the National Academy of Sciences of the United States of America·M DownesR M Evans
Sep 26, 2000·Molecular and Cellular Biology·M KippF O Fackelmayer
Nov 4, 2000·Nature Genetics·S C TsaiE Seto
Jan 4, 2001·The Journal of Biological Chemistry·C A JohnsonB M Turner

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Citations

Apr 22, 2008·Journal of Cellular Biochemistry·Amanda Gonçalves Dos Santos SilvaSabine Mai
Feb 7, 2008·American Journal of Human Genetics·Owen J MarshallK H Andy Choo
Jul 9, 2017·Chromosoma·Oscar MolinaWilliam C Earnshaw

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