Effects of seeding procedures and water quality on recovery of Cryptosporidium oocysts from stream water by using U.S. Environmental Protection Agency Method 1623

Applied and Environmental Microbiology
Donna S FrancyFrank W Schaefer

Abstract

U.S. Environmental Protection Agency method 1623 is widely used to monitor source waters and drinking water supplies for Cryptosporidium oocysts. Matrix spikes, used to determine the effect of the environmental matrix on the method's recovery efficiency for the target organism, require the collection and analysis of two environmental samples, one for analysis of endemic oocysts and the other for analysis of recovery efficiency. A new product, ColorSeed, enables the analyst to determine recovery efficiency by using modified seeded oocysts that can be differentiated from endemic organisms in a single sample. Twenty-nine stream water samples and one untreated effluent sample from a cattle feedlot were collected in triplicate to compare modified seeding procedures to conventional seeding procedures that use viable, unmodified oocysts. Significant negative correlations were found between the average oocyst recovery and turbidity or suspended sediment; this was especially apparent in samples with turbidities greater than 100 nephelometric turbidity units and suspended sediment concentrations greater than 100 mg/liter. Cryptosporidium oocysts were found in 16.7% of the unseeded environmental samples, and concentrations, adjusted for r...Continue Reading

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Nov 11, 2003·Journal of Microbiological Methods·Michael W WareFrank W Schaefer

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Citations

Aug 11, 2007·Archives of Environmental Contamination and Toxicology·Tham C HoangStephen J Klaine
Dec 25, 2012·Applied and Environmental Microbiology·Donna S FrancyH D Alan Lindquist
Aug 2, 2007·Canadian Journal of Microbiology·Zia BukhariFrank W Schaefer
Oct 22, 2005·Letters in Applied Microbiology·M W Ware, F W Schaefer
Feb 27, 2009·Journal of Environmental Quality·Vincent R HillJames E Amburgey
Jun 22, 2021·Journal of Water and Health·Natália de Melo Nasser FavaLyda Patricia Sabogal-Paz

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