Effects of sensitization to dinitrochlorobenzene (DNCB) on clinical pathology parameters and mitogen-mediated blastogenesis in cynomolgus monkeys (Macaca fascicularis)

Toxicologic Pathology
P J BugelskiD G Morgan

Abstract

Immunotoxicologic testing of drug candidates and environmental contaminants is of growing importance. Cutaneous delayed type hypersensitivity (DTH) is a convenient way of testing immune function in vivo. However, DTH testing must not interfere with interpretation of other relevant parameters. We have evaluated the effects of sensitization and challenge with dinitrochlorobenzene (DNCB) on clinical parameters routinely evaluated in toxicity testing and on lectin-mediated blastogenesis. Female cynomolgus monkeys were sensitized to DNCB with 4 daily applications of DNCB in acetone to the skin of the axilla. Fifteen days later, the monkeys were challenged for DTH by applying DNCB to the antecubital skin. Skin fold thickness was measured and the macroscopic appearance of the challenge site was scored 24 and 48 hr after challenge. All 5 monkeys were successfully sensitized to DNCB. There was a significant increase in the mean skin fold thickness (compared to pre-challenge thickness) of 2 mm at 24 hr and 1 mm at 48 hr (p less than 0.001). The clinical score of the challenge site was also increased. Histologic examination of the sensitization and challenge sites from a second group of monkeys exposed to DNCB in an identical manner showe...Continue Reading

References

Mar 1, 1985·The Journal of Investigative Dermatology·R L Bronaugh, H I Maibach
Jan 1, 1983·International Archives of Allergy and Applied Immunology·M TatsumiS Yamazaki
Nov 1, 1984·Annals of Internal Medicine·D J CohenT B Strom
Apr 1, 1984·Clinical Immunology and Immunopathology·L V ChalifouxN L Letvin
Apr 1, 1983·European Journal of Immunology·N L LetvinS F Schlossman
Oct 23, 1980·The New England Journal of Medicine·E R Unanue
Jan 1, 1981·Journal of Medical Primatology·D P Humber, C M Hetherington
Jan 1, 1981·Journal of Medical Primatology·R W FerraresiL D Shott

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Citations

Aug 22, 2012·Journal of Toxicologic Pathology·Caroline BouchezJacques Descotes
Oct 30, 2008·Journal of Immunotoxicology·Helen G Haggerty

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